Recombinant NY-ESO-1 cancer antigen: Production and purification under cGMP conditions

被引：32
|
作者：
Murphy, R [1 ]
Green, S
Ritter, G
Cohen, L
Ryan, D
Woods, W
Rubira, M
Cebon, J
Davis, ID
Sjolander, A
Kypridis, A
Kalnins, H
McNamara, M
Moloney, MB
Ackland, J
Cartwright, G
Rood, J
Dumsday, G
Healey, K
Maher, D
Maraskovsky, E
Chen, YT
Hoffman, EW
Old, LJ
Scott, AM
机构：
[1] Austin Hosp, Biol Prod Facil, Ludwig Inst Canc Res, Melbourne Tumour Biol Branch, Heidelberg, Vic 3084, Australia
[2] CSL Ltd, Parkville, Vic, Australia
[3] Ludwig Inst Canc Res, New York Branch, New York, NY USA
来源：
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2005年 / 35卷 / 02期
关键词：
NY-ESO-1; cGMP; cancer-testis antigen; recombinant protein;
D O I：
10.1081/PB-200054732
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The cancer-testis antigen, NY-ESO-1, has been engineered into a bacterial expression plasmid which incorporates a His(6)-tag. The plasmid was transfected into E. coli strain BL21 and Master and Working cell banks generated from this expression system. Three 15-litre fermentations were performed under cGMP (code of Good Manufacturing Practice) conditions and the crude NY-ESO-1 tagged protein isolated as solubilised inclusion bodies. A three-step cGMP chromatography process (immobilised metal affinity, anion exchange, and hydrophobic interaction) was utilised to purify the protein. The purified NY-ESO-1 is being used in early stage human cancer vaccine trials in Australia and the U.S.A.
引用
收藏
页码：119 / 134
页数：16
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