Antioxidant, cytotoxic and apoptotic activities of the rhizome of Zingiber zerumbet Linn. in Ehrlich ascites carcinoma bearing Swiss albino mice

被引:9
|
作者
Ali, Hanif [1 ]
Hasi, Rumana Yesmin [1 ]
Islam, Majidul [1 ]
Haque, Md Shajedul [1 ]
Alkhanani, Mustfa F. [2 ]
Almalki, Atiah H. [3 ,4 ]
Haque, Shafiul [5 ,6 ]
Sayyed, R. Z. [7 ,8 ]
Yeasmin, Tanzima [1 ]
机构
[1] Univ Rajshahi, Dept Biochem & Mol Biol, Rajshahi 6205, Bangladesh
[2] Univ Hafr Al Batin, Coll Sci, Biol Dept, POB 1803, Hafar al Batin 31991, Saudi Arabia
[3] Taif Univ, Coll Pharm, Dept Pharmaceut Chem, POB 11099, Taif 21944, Saudi Arabia
[4] Taif Univ, Coll Pharm, Addict & Neurosci Res Unit, Al Hawiah 21944, Taif, Saudi Arabia
[5] Jazan Univ, Coll Nursing & Allied Hlth Sci, Res & Sci Studies Unit, Jazan 45142, Saudi Arabia
[6] Bursa Uludag Univ, Fac Med, Gorukle Campus, TR-16059 Bursa, Turkey
[7] GB Patel Sci, PSGVP Mandals SI Patil Arts, Dept Microbiol, Shahada 425409, India
[8] STKVS Commerce Coll, Shahada 425409, India
关键词
CELL-CYCLE ARREST; OXIDATIVE STRESS; GROWTH-INHIBITION; FREE-RADICALS; NITRIC-OXIDE; EXTRACT; CANCER; PROLIFERATION; CHEMOPREVENTION; BIOLOGY;
D O I
10.1038/s41598-022-15498-8
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Due to having a long history of traditional uses as a functional food, Zingiber zerumbet was selected here to explore the inherent antioxidant and antineoplastic activities of methanolic extract of its rhizome (MEZZR) against Ehrlich ascites carcinoma (EAC) cells. The rich polyphenol containing MEZZR showed a marked DPPH, ABTS, nitric oxide radicals and lipid peroxidation inhibition activity with an IC50 of 3.43 +/- 1.25, 11.38 +/- 1.39, 23.12 +/- 3.39 and 16.47 +/- 1.47 mu g/ml, respectively, when compared to the standard catechin. In vivo, MEZZR significantly inhibited EAC cell growth, decreased body weight gain, increased life span and restored the altered hematological characteristics of EAC-bearing mice. Moreover, MEZZR induced nuclear condensation and fragmentation, which are notable features of apoptosis as observed by fluorescence microscopy after staining EAC cells of MEZZR-treated mice with Hoechst 33342. Additionally, in vitro, the cell growth inhibition caused by the MEZZR in MTT assay, was remarkably decreased in the presence of caspase-3, -8 and -9 inhibitors. This study thus suggests that MEZZR may possess promising antiproliferative efficacy against EAC cells by inducing cell apoptosis.
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页数:11
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