Use of the TRP1 auxotrophic marker for gene disruption and phenotypic analysis in yeast:: a note of warning

被引：18

作者：

Gonzalez, Asier ^{[1
,2
]}

Larroy, Carolina ^{[1
]}

Biosca, Josep A. ^{[1
]}

Arino, Joaquin ^{[1
,2
]}

机构：

[1] Univ Autonoma Barcelona, Dept Bioquim & Biol Mol, E-08193 Barcelona, Spain

[2] Univ Autonoma Barcelona, Inst Biotecnol & Biomed, E-08193 Barcelona, Spain

来源：

FEMS YEAST RESEARCH | 2008年 / 8卷 / 01期

关键词：

phenotypic analysis; auxotrophic marker; TRP1; gene;

D O I：

10.1111/j.1567-1364.2007.00315.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The TRP1 marker has been commonly used for gene disruption experiments and subsequent phenotypic analysis. However, introduction of the TRP1 gene into a trpl strain markedly affects growth under many conditions used for phenotypic profiling. Therefore, its use in the past should be revisited and utilization of this marker should be avoided in future analyses.

引用

页码：2 / 5

页数：4

共 22 条

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