Evaluation of MALDI-TOF MS as a tool for detection of Listeria spp. directly from selective enrichment broth from food and stool samples

被引：5
|
作者：
Campos Araujo, Thais Martins [1 ]
Lisboa Pereira, Rodrigo de Castro [1 ]
Ribeiro Freitag, Isabelle Geoffroy [1 ]
Rusak, Leonardo Alves [2 ]
Batista Botelho, Larissa Alvarenga [3 ]
Hofer, Ernesto [1 ]
Hofer, Cristina Barroso [4 ]
Vallim, Deyse Christina [1 ]
机构：
[1] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Zoonoses Bacterianas, Setor Listeria, Av Brasil 4365,Pavilhao Rocha Lima,3 Andar, Rio De Janeiro, RJ, Brazil
[2] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Fisiol Bacteriana, Rio De Janeiro, RJ, Brazil
[3] Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo de Goes, Lab Investi Microbiol Med, Rio De Janeiro, RJ, Brazil
[4] Univ Fed Rio de Janeiro, Fac Med, Programa Posgrad Doencas Infecciosas & Parasitari, Rio De Janeiro, RJ, Brazil
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2020年 / 173卷
关键词：
Enrichment broth; Listeria spp; Listeria monocytogenes; MALDI-TOF MS; Foodborne pathogen detection; DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; RAPID IDENTIFICATION; MONOCYTOGENES; BACTERIA;
D O I：
10.1016/j.mimet.2020.105936
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We evaluated the detection of Listeria spp. using MALDI-TOF MS directly in enrichment broths, without isolated colonies, with naturally contaminated food and stool samples. The success rate was 77%. Considering the reduced time for diagnosis and the success rate, this is a promising screening tool, but more tests are needed to determine its viability.
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页数：3
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