FACS and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver

被引：2
|
作者：
Braun, Tatum W. [1 ]
Kuoch, Michael K. [1 ]
Khandros, Eugene [2 ]
Li, Hojun [1 ,3 ,4 ]
机构：
[1] MIT, Koch Inst Integrat Canc Res, Cambridge, MA 02142 USA
[2] Childrens Hosp Philadelphia, Div Hematol, Philadelphia, PA 19104 USA
[3] Dana Farber Boston Childrens Hosp, Canc & Blood Disorders Ctr, Boston, MA 02215 USA
[4] Harvard Med Sch, Dept Pediat, Boston, MA 02215 USA
来源：
STAR PROTOCOLS | 2022年 / 3卷 / 01期
关键词：
SELF-RENEWAL; BFU-E;
D O I：
10.1016/j.xpro.2021.101070
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Early erythroid progenitors are transit-amplifying cells with high proliferative ca-pacity committed to undergoing red cell differentiation. CD71/CD24low progen-itors are less mature and have greater proliferative capacity than CD71/ CD24high. We present protocols for isolation of CD71/CD24low progenitors from mouse fetal liver using both fluorescence-activated cell sorting (FACS) and immunomagnetic enrichment. CD71/CD24low progenitors isolated with both approaches show similar transcriptomes at single-cell resolution and exhibit characteristic proliferative responses to glucocorticoids. For complete details on the use and execution of this protocol, please refer to Li et al. (2019).
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页数：18
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