An anti-G protein scintillation proximity assay for M1-M5 receptor-mediated GTPγS binding.

被引:0
|
作者
McKinzie, JH [1 ]
Sawyer, BD [1 ]
Felder, CC [1 ]
Falcone, J [1 ]
Silbernagel, A [1 ]
McClure, D [1 ]
DeLapp, NW [1 ]
机构
[1] Eli Lilly & Co, Lilly Res Labs, Indianapolis, IN 46285 USA
关键词
D O I
10.1016/S0024-3205(99)90495-2
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
32
引用
收藏
页码:570 / 570
页数:1
相关论文
共 50 条
  • [1] Muscarinic acetylcholine receptor-mediated activation of Gq in rat brain membranes determined by guanosine-5'-O-(3-[35S]thio)triphosphate ([35S]GTPγS) binding using an anti-G protein scintillation proximity assay
    Odagaki, Yuji
    Toyoshima, Ryoichi
    JOURNAL OF NEURAL TRANSMISSION, 2012, 119 (05) : 525 - 532
  • [2] Muscarinic acetylcholine receptor-mediated activation of Gq in rat brain membranes determined by guanosine-5′-O-(3-[35S]thio)triphosphate ([35S]GTPγS) binding using an anti-G protein scintillation proximity assay
    Yuji Odagaki
    Ryoichi Toyoshima
    Journal of Neural Transmission, 2012, 119 : 525 - 532
  • [3] A study of M1 receptor coupling to inhibitory G proteins in CHO cells via GTPγS binding determined with anti-G protein antibodies.
    DeLapp, NW
    McClure, D
    McKinzie, J
    Silbernagel, A
    Sawyer, BD
    Felder, CC
    LIFE SCIENCES, 1999, 64 (6-7) : 569 - 569
  • [4] Comparison of GTP-γ-35S and 3H-NMS binding in CHO cells stably expressing human m1-m5 muscarinic receptors using scintillation proximity assays
    Sawyer, BD
    Silbernagel, A
    McKinzie, JB
    Falcone, J
    Delapp, N
    Felder, CC
    LIFE SCIENCES, 1999, 64 (6-7) : 568 - 568
  • [5] Radioligand binding assay of M1-M5 muscarinic cholinergic receptor subtypes in human peripheral blood lymphocytes
    Tayebati, SK
    Codini, M
    Gallai, V
    Mannino, F
    Parnetti, L
    Ricci, A
    Sarchielli, P
    Amenta, F
    JOURNAL OF NEUROIMMUNOLOGY, 1999, 99 (02) : 224 - 229
  • [6] Characterization of the kappa opioid receptor-mediated G protein signaling in mouse striatum using a [35S] GTPγS binding assay
    Zhou, Lei
    Stahl, Edward
    Lovell, Kimberly
    Slauson, Stephen
    Frankowski, Kevin
    Aube, Jeffrey
    Bohn, Laura
    FASEB JOURNAL, 2014, 28 (01):
  • [7] 5-HT1A receptor-mediated G protein activation assessed by [35S]GTPγS binding in rat cerebral cortex
    Odagaki, Y
    Toyoshima, R
    EUROPEAN JOURNAL OF PHARMACOLOGY, 2005, 521 (1-3) : 49 - 58
  • [8] Ionic strength of assay buffers influences antagonist binding affinity estimates at muscarinic M1-M5 cholinoceptors
    Loury, DN
    Hegde, SS
    Bonhaus, DW
    Eglen, RM
    LIFE SCIENCES, 1999, 64 (6-7) : 557 - 557
  • [9] M1 and M5 muscarinic acetylcholine receptor-mediated leukocyte migration
    Harada, Kosuke
    Matsumoto, Yuji
    Umemura, Kazuo
    JOURNAL OF PHARMACOLOGICAL SCIENCES, 2012, 118 : 173P - 173P
  • [10] Characterization of 5-HT1A receptor-mediated [35S]GTPγS binding in rat hippocampal membranes
    Alper, RH
    Nelson, DL
    EUROPEAN JOURNAL OF PHARMACOLOGY, 1998, 343 (2-3) : 303 - 312