Exploring Ferredoxin-Dependent Glutamate Synthase as an Enzymatic Bioelectrocatalyst

被引：28
|
作者：
Wu, Fei [1 ,2 ,3 ]
Yu, Ping [1 ,2 ,3 ]
Yang, Xiaoti [1 ,2 ,3 ]
Han, Zhongjie [1 ]
Wang, Ming [1 ,2 ,3 ]
Mao, Lanqun [1 ,2 ,3 ]
机构：
[1] Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
[2] Univ CAS, Beijing 100049, Peoples R China
[3] CAS Res Educ Ctr Excellence Mol Sci, Beijing 100190, Peoples R China
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2018年 / 140卷 / 40期
基金：
美国国家科学基金会;
关键词：
IRON-SULFUR FLAVOPROTEIN; SP PCC 6803; AZOSPIRILLUM-BRASILENSE; OXIDATION-REDUCTION; NITROGENASE; EXPRESSION; ENZYMES; CLONING; CELL; CO2;
D O I：
10.1021/jacs.8b08020
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Ferredoxin-dependent glutamate synthase (Fd-GltS) is reported as an enzymatic bioelectrocatalyst for the first time. By configuring mediated electrochemical interfaces with mediators of different redox potentials, we realize bioelectrosynthesis or bioelectrooxidation of glutamate with recombinant Fd-GltS from cyanobacteria. Particularly, bioelectrocatalytic oxidation of glutamate by Fd-GltS is demonstrated to be oxygen independent. This study reinforces a new catalytic option for developing enzymatic bioelectronic devices for powering, sensing or synthesis.
引用
收藏
页码：12700 / 12704
页数：5
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