Budding yeast Dsk2 protein forms a homodimer via its C-terminal UBA domain

被引：25
|
作者：
Sasaki, T
Funakoshi, M
Endicott, JA
Kobayashi, H [1 ]
机构：
[1] Kyushu Univ, Grad Sch Med Sci, Dept Mol Biol, Fukuoka 8128582, Japan
[2] Univ Oxford, Dept Biochem, Oxford OX1 3QU, England
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 336卷 / 02期
关键词：
Dsk2; UBA domain; dimerization; polyubiquitin; ubiquitin-proteasome pathway;
D O I：
10.1016/j.bbrc.2005.08.126
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Budding yeast Dsk2 is a family of UbL-UBA proteins that can interact with both polyubiquitin and the proteasome, and is thereby thought to function as a shuttle protein in the ubicluitin-proteasome pathway. Here we show that Dsk2 can homodimerize via its C-terminal UBA domain in the absence of ubiquitin. Dsk2 mutants defective in the UBA domain do not dimerize and do not bind polyubiquitin. The expression of Dsk2 UBA mutants fails to restore the growth defect caused by DSK2 disruption although that of wild-type Dsk2 can restore the defect. These results suggest that Dsk2 homodimerization via the UBA domain plays a role in regulating polyubiquitin binding in the ubiquitin-proteasome pathway. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码：530 / 535
页数：6
相关论文
共 50 条
  • [1] Ubiquitin chains in the Dsk2 UBL domain mediate Dsk2 stability and protein degradation in yeast
    Sekiguchi, Takeshi
    Sasaki, Toru
    Funakoshi, Minoru
    Ishii, Takashi
    Saitoh, Yoh-hei
    Kaneko, Shu-ichi
    Kobayashi, Hideki
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2011, 411 (03) : 555 - 561
  • [2] The C-Terminal Domain of the MutL Homolog from Neisseria gonorrhoeae Forms an Inverted Homodimer
    Namadurai, Sivakumar
    Jain, Deepti
    Kulkarni, Dhananjay S.
    Tabib, Chaitanya R.
    Friedhoff, Peter
    Rao, Desirazu N.
    Nair, Deepak T.
    PLOS ONE, 2010, 5 (10):
  • [3] Formation of stable homodimer via the C-terminal α-helical domain of coronavirus nonstructural protein 9 is critical for its function in viral replication
    Chen, Bo
    Fang, Shouguo
    Tam, James P.
    Liu, Ding Xiang
    VIROLOGY, 2009, 383 (02) : 328 - 337
  • [4] A mechanism of ACK1 activation in cancer via C-terminal UBA domain truncation
    Balasooriya, Eranga
    Eastmond, Reillu
    Madusanka, Deshan
    Owen, Jacob
    Gashler, Jack
    Lopez-Palacios, Tania
    Andersen, Joshua
    FASEB JOURNAL, 2022, 36
  • [5] Repeat-Specific Functions for the C-Terminal Domain of RNA Polymerase II in Budding Yeast
    Babokhov, Michael
    Mosaheb, Mohammad M.
    Baker, Richard W.
    Fuchs, Stephen M.
    G3-GENES GENOMES GENETICS, 2018, 8 (05): : 1593 - 1601
  • [6] Apolipoprotein(a) binds to proteoglycan decorin via its C-terminal domain
    Klezovitch, ON
    Edelstein, C
    Scanu, AM
    CIRCULATION, 1997, 96 (08) : 627 - 627
  • [7] Escherichia coli RelA Regulation via Its C-Terminal Domain
    Kaspy, Ilana
    Glaser, Gad
    FRONTIERS IN MICROBIOLOGY, 2020, 11
  • [8] Role of C-Terminal Region of Yeast ADP/ATP Carrier 2 Protein: Dynamics of Flexible C-Terminal Arm
    Ohkura, Kazuto
    Hori, Hitoshi
    Shinohara, Yasuo
    ANTICANCER RESEARCH, 2009, 29 (11) : 4897 - 4900
  • [9] Dimerization of Sir3 via its C-terminal winged helix domain is essential for yeast heterochromatin formation
    Oppikofer, Mariano
    Kueng, Stephanie
    Keusch, Jeremy J.
    Hassler, Markus
    Ladurner, Andreas G.
    Gut, Heinz
    Gasser, Susan M.
    EMBO JOURNAL, 2013, 32 (03): : 437 - 449
  • [10] Chibby forms a homodimer through a heptad repeat of leucine residues in its C-terminal coiled-coil motif
    Mofunanya, Adaobi
    Li, Feng-Qian
    Hsieh, Jen-Chih
    Takemaru, Ken-Ichi
    BMC MOLECULAR BIOLOGY, 2009, 10
12345