Histochemical staining of cadmium with 2-(8-quinolylazo)-4,5-diphenylimidazole

被引:0
|
作者
Sumi, Y [1 ]
Itoh, MT [1 ]
Muraki, T [1 ]
Suzuki, T [1 ]
机构
[1] ST MARIANNA UNIV,SCH MED,DEPT ANAT,MIYAMAE KU,KAWASAKI,KANAGAWA 216,JAPAN
来源
HISTOCHEMISTRY AND CELL BIOLOGY | 1996年 / 106卷 / 02期
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中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In order to develop a stain with increased sensitivity and selectivity for cadmium (Cd), we synthesized and characterized 2-(8-quinolylazo)-4,5-diphenylimidazole (QAI). This chelating agent was more than twice as sensitive for Cd than the best conventional staining agents, including benzothiazolylazo-beta-naphthol. Differentiation between Cd and zinc (Zn) was achieved by immersing tissue sections in TRIS(2-aminoethyl)amine before they were stained with QAI. This pretreatment made it possible to selectively stain for Cd by blocking Zn.
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页码:223 / 227
页数:5
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