Infection and Rapid Transmission of SARS-CoV-2 in Ferrets

被引：653

作者：

Kim, Young-Il ^{[1
,2
,3
]}

Kim, Seong-Gyu ^{[1
,2
]}

Kim, Se-Mi ^{[1
,2
]}

Kim, Eun-Ha ^{[1
,2
,3
]}

Park, Su-Jin ^{[1
,2
,3
]}

Yu, Kwang-Min ^{[1
,2
,3
]}

Chang, Jae-Hyung ^{[1
,2
]}

Kim, Eun Ji ^{[1
,2
]}

Lee, Seunghun ^{[1
,2
]}

Casel, Mark Anthony B. ^{[1
,2
,3
]}

Um, Jihye ^{[5
]}

Song, Min-Suk ^{[1
,2
,3
]}

Jeong, Hye Won ^{[1
,2
]}

Lai, Van Dam ^{[4
]}

Kim, Yeonjae ^{[5
]}

Chin, Bum Sik ^{[5
]}

Park, Jun-Sun ^{[5
]}

Chung, Ki-Hyun ^{[5
]}

Foo, Suan-Sin ^{[6
]}

Poo, Haryoung ^{[7
]}

Mo, In-Pil ^{[4
]}

Lee, Ok-Jun ^{[1
,2
]}

Webby, Richard J. ^{[8
]}

Jung, Jae U. ^{[6
]}

Choi, Young Ki ^{[1
,2
,3
]}

机构：

[1] Chungbuk Natl Univ, Coll Med, Cheongju, South Korea

[2] Chungbuk Natl Univ, Med Res Inst, Cheongju, South Korea

[3] Chungbuk Natl Univ, Zoonot Infect Dis Res Ctr, Cheongju, South Korea

[4] Chungbuk Natl Univ, Coll Vet Med, Cheongju, South Korea

[5] Natl Med Ctr, Res Inst Publ Hlth, Seoul, South Korea

[6] Univ Southern Calif, Keck Sch Med, Dept Mol Microbiol & Immunol, Los Angeles, CA 90033 USA

[7] Univ Sci & Technol, Infect Dis Res Ctr, Korea Res Inst Biosci & Biotechnol, Daejeon, South Korea

[8] St Jude Childrens Res Hosp, Dept Infect Dis, Div Virol, Memphis, TN 38105 USA

来源：

CELL HOST & MICROBE | 2020年 / 27卷 / 05期

基金：

新加坡国家研究基金会;

关键词：

RESPIRATORY SYNDROME SARS; CORONAVIRUS; VIRUS; MODELS;

D O I：

10.1016/j.chom.2020.03.023

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in China and rapidly spread worldwide. To prevent SARS-CoV-2 dissemination, understanding the in vivo characteristics of SARS-CoV-2 is a high priority. We report a ferret model of SARS-CoV-2 infection and transmission that recapitulates aspects of human disease. SARS-CoV-2-infected ferrets exhibit elevated body temperatures and virus replication. Although fatalities were not observed, SARS-CoV-2-infected ferrets shed virus in nasal washes, saliva, urine, and feces up to 8 days post-infection. At 2 days post-contact, SARS-CoV-2 was detected in all naive direct contact ferrets. Furthermore, a few naive indirect contact ferrets were positive for viral RNA, suggesting airborne transmission. Viral antigens were detected in nasal turbinate, trachea, lungs, and intestine with acute bronchiolitis present in infected lungs. Thus, ferrets represent an infection and transmission animal model of COVID-19 that may facilitate development of SARS-CoV-2 therapeutics and vaccines.

引用

页码：704 / +

页数：8

共 50 条

[1] Influenza Infection in Ferrets with SARS-CoV-2 Infection History
Boas de Melo, Caroline Vilas
Peters, Florence
van Dijken, Harry
Lenz, Stefanie
van de Ven, Koen
Wijsman, Lisa
Gomersbach, Angela
Schouten, Tanja
van Kasteren, Puck B.
van den Brand, Judith
de Jonge, Jorgen
[J]. MICROBIOLOGY SPECTRUM, 2022, 10 (06):
[2] SARS-CoV-2 in ferrets
Middlemiss, Christine
Voas, Sheila
Glossop, Christianne
Huey, Robert
[J]. VETERINARY RECORD, 2021, 188 (02) : 133 - 133
[3] Characterisation and natural progression of SARS-CoV-2 infection in ferrets
Au, Gough G.
Marsh, Glenn A.
McAuley, Alexander J.
Lowther, Suzanne
Trinidad, Lee
Edwards, Sarah
Todd, Shawn
Barr, Jennifer
Bruce, Matthew P.
Poole, Timothy B.
Brown, Sheree
Layton, Rachel
Riddell, Sarah
Rowe, Brenton
Soldani, Elisha
Suen, Willy W.
Bergfeld, Jemma
Bingham, John
Payne, Jean
Durr, Peter A.
Drew, Trevor W.
Vasan, Seshadri S.
[J]. SCIENTIFIC REPORTS, 2022, 12 (01)
[4] Natural SARS-CoV-2 Infection in Kept Ferrets, Spain
Gortazar, Christian
Barroso-Arevalo, Sandra
Ferreras-Colino, Elisa
Isla, Julio
de la Fuente, Gabriela
Rivera, Belen
Dominguez, Lucas
de la Fuente, Jose
Sanchez-Vizcaino, Jose M.
[J]. EMERGING INFECTIOUS DISEASES, 2021, 27 (07) : 1994 - 1996
[5] Characterisation and natural progression of SARS-CoV-2 infection in ferrets
Gough G. Au
Glenn A. Marsh
Alexander J. McAuley
Suzanne Lowther
Lee Trinidad
Sarah Edwards
Shawn Todd
Jennifer Barr
Matthew P. Bruce
Timothy B. Poole
Sheree Brown
Rachel Layton
Sarah Riddell
Brenton Rowe
Elisha Soldani
Willy W. Suen
Jemma Bergfeld
John Bingham
Jean Payne
Peter A. Durr
Trevor W. Drew
Seshadri S. Vasan
[J]. Scientific Reports, 12
[6] No indication for SARS-CoV-2 transmission to pet ferrets, in five cities in Poland, 2021 - antibody testing among ferrets living with owners infected with SARS-CoV-2 or free of infection
Edyta Kaczorek-Łukowska
Kerstin Wernike
Martin Beer
Alicja Blank
Joanna Małaczewska
Mirosława Blank
Anna Jałonicka
Andrzej Krzysztof Siwicki
[J]. Acta Veterinaria Scandinavica, 65
[7] Intranasal Infection of Ferrets with SARS-CoV-2 as a Model for Asymptomatic Human Infection
Everett, Helen E.
Lean, Fabian Z. X.
Byrne, Alexander M. P.
van Diemen, Pauline M.
Rhodes, Shelley
James, Joe
Mollett, Benjamin
Coward, Vivien J.
Skinner, Paul
Warren, Caroline J.
Bewley, Kevin R.
Watson, Samantha
Hurley, Shellene
Ryan, Kathryn A.
Hall, Yper
Simmons, Hugh
Nunez, Alejandro
Carroll, Miles W.
Brown, Ian H.
Brookes, Sharon M.
[J]. VIRUSES-BASEL, 2021, 13 (01):
[8] Transplacental transmission of SARS-CoV-2 infection
Alexandre J. Vivanti
Christelle Vauloup-Fellous
Sophie Prevot
Veronique Zupan
Cecile Suffee
Jeremy Do Cao
Alexandra Benachi
Daniele De Luca
[J]. Nature Communications, 11
[9] Transplacental transmission of SARS-CoV-2 infection
Vivanti, Alexandre J.
Vauloup-Fellous, Christelle
Prevot, Sophie
Zupan, Veronique
Suffee, Cecile
Do Cao, Jeremy
Benachi, Alexandra
De Luca, Daniele
[J]. NATURE COMMUNICATIONS, 2020, 11 (01)
[10] Age-Related Susceptibility of Ferrets to SARS-CoV-2 Infection
Martins, Mathias
Fernandes, Maureen H., V
Joshi, Lok R.
Diel, Diego G.
[J]. JOURNAL OF VIROLOGY, 2022, 96 (03)

← 1 2 3 4 5 →