Implementation of a Raspberry-Pi-based LED array microscope for multi-contrast images

被引:1
|
作者
Maruyama, Ryoji [1 ]
Shimada, Tomoki [1 ]
Yokoe, Ryota [1 ]
Arimoto, Hidenobu [2 ]
Watanabe, Wataru [1 ]
机构
[1] Ritsumeikan Univ, Coll Sci & Engn, Dept Elect & Elect Engn, 1-1-1 Nojihigashi, Kusatsu, Shiga 5258577, Japan
[2] Natl Inst Adv Ind Sci & Technol, Elect & Photon Res Inst, AIST Tsukuba Cent 5,1-1-1 Higashi, Tsukuba, Ibaraki 3058565, Japan
来源
关键词
bright-field image; dark-field image; differential phase contrast image; multi-contrast image; LED array microscopy; Raspberry pi; DIFFERENTIAL PHASE-CONTRAST; SPLIT DETECTOR;
D O I
10.1117/12.2319076
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
The light emitting diode (LED) array microscope enables various multi-contrast imaging such as bright-field, dark-field and differential phase-contrast by various illumination patterns without any expensive optical components. We build an LED array microscopic system operated with Raspberry Pi. Illumination patterns are controlled with Raspberry Pi and images are obtained with a Raspberry Pi camera module. We demonstrate acquisition of bright-field, dark-field, and differential phase-contrast of cells.
引用
收藏
页数:4
相关论文
共 50 条
  • [1] Multi-Contrast Imaging and Digital Refocusing on a Mobile Microscope with a Domed LED Array
    Phillips, Zachary F.
    D'Ambrosio, Michael V.
    Tian, Lei
    Rulison, Jared J.
    Patel, Hurshal S.
    Sadras, Nitin
    Gande, Aditya V.
    Switz, Neil A.
    Fletcher, Daniel A.
    Waller, Laura
    [J]. PLOS ONE, 2015, 10 (05):
  • [2] LED array reflectance microscopy for scattering-based multi-contrast imaging
    Song, Weiye
    Matlock, Alex
    Fu, Sipei
    Qin, Xiaodan
    Feng, Hui
    Gabel, Christopher, V
    Tian, Lei
    Yi, Ji
    [J]. OPTICS LETTERS, 2020, 45 (07) : 1647 - 1650
  • [3] An Intelligent Raspberry-Pi-Based Parking Slot Identification System
    Agarwal, Raghav
    Sharma, Gaurav
    Singh, Nirdesh
    Nair, Hrishikesh S
    Daga, Yash
    Lakshmi, D. Venkata
    [J]. EAI Endorsed Transactions on Industrial Networks and Intelligent Systems, 2023, 10 (04)
  • [4] Miniaturized multi-contrast quantitative phase imaging microscope
    Fan, Yao
    Ding, Junyi
    Sun, Jiasong
    Chen, Qian
    Zuo, Chao
    [J]. OPTICS FRONTIER ONLINE 2020: OPTICS IMAGING AND DISPLAY, 2020, 11571
  • [5] Smartphone-based multi-contrast microscope using color-multiplexed illumination
    Daeseong Jung
    Jun-Ho Choi
    Soocheol Kim
    Suho Ryu
    Wonchan Lee
    Jong-Seok Lee
    Chulmin Joo
    [J]. Scientific Reports, 7
  • [6] Smartphone-based multi-contrast microscope using color-multiplexed illumination
    Jung, Daeseong
    Choi, Jun-Ho
    Kim, Soocheol
    Ryu, Suho
    Lee, Wonchan
    Lee, Jong-Seok
    Joo, Chulmin
    [J]. SCIENTIFIC REPORTS, 2017, 7
  • [7] A miniature multi-contrast microscope for functional imaging in freely behaving animals
    Senarathna, Janaka
    Yu, Hang
    Deng, Callie
    Zou, Alice L.
    Issa, John B.
    Hadjiabadi, Darian H.
    Gil, Stacy
    Wang, Qihong
    Tyler, Betty M.
    Thakor, Nitish, V
    Pathak, Arvind P.
    [J]. NATURE COMMUNICATIONS, 2019, 10 (1)
  • [8] A miniature multi-contrast microscope for functional imaging in freely behaving animals
    Janaka Senarathna
    Hang Yu
    Callie Deng
    Alice L. Zou
    John B. Issa
    Darian H. Hadjiabadi
    Stacy Gil
    Qihong Wang
    Betty M. Tyler
    Nitish V. Thakor
    Arvind P. Pathak
    [J]. Nature Communications, 10
  • [9] Fast Recovery of Compressed Multi-Contrast Magnetic Resonance Images
    Gungor, Alper
    Kopanoglu, Emre
    Cukur, Tolga
    Guven, H. Emre
    [J]. MEDICAL IMAGING 2017: IMAGE PROCESSING, 2017, 10133
  • [10] Real-time multiple objects tracking on Raspberry-Pi-based smart embedded camera
    Dziri, Aziz
    Duranton, Marc
    Chapuis, Roland
    [J]. JOURNAL OF ELECTRONIC IMAGING, 2016, 25 (04)