Relationship of Late Lactation Milk Somatic Cell Count and Cathelicidin with Intramammary Infection in Small Ruminants

被引:5
|
作者
Puggioni, Giulia Maria Grazia [1 ]
Tedde, Vittorio [1 ]
Uzzau, Sergio [1 ]
Dore, Simone [2 ]
Liciardi, Manuele [2 ]
Cannas, Eugenia Agnese [2 ]
Pollera, Claudia [3 ]
Moroni, Paolo [3 ,4 ]
Bronzo, Valerio [3 ]
Addis, Maria Filippa [1 ,3 ]
机构
[1] Porto Conte Ric, I-07041 Porto, Italy
[2] Natl Reference Ctr Sheep & Goat Mastitis, Ist Zooprofilatt Sperimentale Sardegna, Via Duca Abruzzi, I-07100 Sassari, Italy
[3] Univ Milan, Dept Vet Med, Via Univ 6, I-26900 Lodi, Italy
[4] Cornell Univ, Qual Milk Prod Serv, 240 Farrier Rd, Ithaca, NY 14853 USA
来源
PATHOGENS | 2020年 / 9卷 / 01期
关键词
subclinical mastitis; sheep; goat; late lactation; cathelicidin ELISA; somatic cell count; bacteriological culture; MAMMARY EPITHELIAL-CELLS; CALIFORNIA MASTITIS TEST; SUBCLINICAL MASTITIS; DAIRY GOATS; SHEEP; DIAGNOSIS; PROTEINS; RELEASE;
D O I
10.3390/pathogens9010037
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Late lactation is a critical moment for making mastitis management decisions, but in small ruminants the reliability of diagnostic tests is typically lower at this stage. We evaluated somatic cell counts (SCC) and cathelicidins (CATH) in late lactation sheep and goat milk for their relationship with intramammary infections (IMI), as diagnosed by bacteriological culture (BC). A total of 315 sheep and 223 goat half-udder milk samples collected in the last month of lactation were included in the study. IMI prevalence was 10.79% and 15.25%, respectively, and non-aureus staphylococci were the most common finding. Taking BC as a reference, the diagnostic performance of SCC and CATH was quite different in the two species. In sheep, receiver operating characteristic (ROC) analysis produced a higher area under the curve (AUC) value for CATH than SCC (0.9041 versus 0.8829, respectively). Accordingly, CATH demonstrated a higher specificity than SCC (82.92% versus 73.67%, respectively) at comparable sensitivity (91.18%). Therefore, CATH showed a markedly superior diagnostic performance than SCC in late lactation sheep milk. In goats, AUC was <0.67 for both parameters, and CATH was less specific than SCC (61.90% versus 65.08%) at comparable sensitivity (64.71%). Therefore, both CATH and SCC performed poorly in late lactation goats. In conclusion, sheep can be screened for mastitis at the end of lactation, while goats should preferably be tested at peak lactation. In late lactation sheep, CATH should be preferred over SCC for its higher specificity, but careful cost/benefit evaluations will have to be made.
引用
收藏
页数:10
相关论文
共 50 条
  • [1] Evaluation of test-day milk somatic cell count to predict intramammary infection in late lactation grazing dairy cows
    Clabby, C.
    Valldecabres, A.
    Dillon, P.
    McParland, S.
    Arkins, S.
    O'Sullivan, K.
    Flynn, J.
    Murphy, J.
    Bolona, P. Silva
    [J]. JOURNAL OF DAIRY SCIENCE, 2023, 106 (07) : 4991 - 5001
  • [2] Associations Between Somatic Cell Count and Intramammary Infection in Early Lactation of Dairy Cows
    Asadpour, R.
    Tayefi-Nasrabadi, H.
    Moghadam, G. A.
    Nofouzi, K.
    [J]. JOURNAL OF ANIMAL AND VETERINARY ADVANCES, 2008, 7 (10): : 1223 - 1226
  • [3] Association between quarter-level milk somatic cell count and intramammary bacterial infection in late-lactation Irish grazing dairy cows
    Valldecabres, Ainhoa
    Clabby, Clare
    Dillon, Pat
    Bolona, Pablo Silva
    [J]. JDS COMMUNICATIONS, 2023, 4 (04):
  • [4] Evaluation of the composite milk somatic cell count as a predictor of intramammary infection in dairy cattle
    Jashari, R.
    Piepers, S.
    De Vliegher, S.
    [J]. JOURNAL OF DAIRY SCIENCE, 2016, 99 (11) : 9271 - 9286
  • [5] Association of Milk Somatic Cell Count with Bacteriological Cure of Intramammary Infection-A Review
    Williamson, Jenna
    Callaway, Todd
    Rollin, Emmanuel
    Ryman, Valerie
    [J]. AGRICULTURE-BASEL, 2022, 12 (09):
  • [6] Physiological Aspects of Milk Somatic Cell Count in Small Ruminants-A Review
    Kaskous, Shehadeh
    Farschtschi, Sabine
    Pfaffl, Michael W.
    [J]. DAIRY, 2023, 4 (01) : 26 - 42
  • [7] Relationship between somatic cell count and milk yield in different stages of lactation
    Hagnestam-Nielsen, C.
    Emanuelson, U.
    Berglund, B.
    Strandberg, E.
    [J]. JOURNAL OF DAIRY SCIENCE, 2009, 92 (07) : 3124 - 3133
  • [8] Relationship between somatic cell count and intramammary infection of the half udder in dairy ewes
    GonzalezRodriguez, MC
    Gonzalo, C
    SanPrimitivo, F
    Carmenes, P
    [J]. JOURNAL OF DAIRY SCIENCE, 1995, 78 (12) : 2753 - 2759
  • [9] Sensitivity and specificity of somatic cell count and California Mastitis Test for identifying intramammary infection in early lactation
    Sargeant, JM
    Leslie, KE
    Shirley, JE
    Pulkrabek, BJ
    Lim, GH
    [J]. JOURNAL OF DAIRY SCIENCE, 2001, 84 (09) : 2018 - 2024
  • [10] SOMATIC CELL COUNTS AND TOTAL BACTERIAL COUNT IN BULK TANK MILK OF SMALL RUMINANTS
    Olechnowicz, Jan
    Jaskowski, Jedrzej Maria
    [J]. SLOVENIAN VETERINARY RESEARCH, 2012, 49 (01) : 13 - 18