Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules

被引：20
|
作者：
Egoshi, Syusuke [1 ]
Dodo, Kosuke [1 ,2 ]
Ohgane, Kenji [1 ]
Sodeoka, Mikiko [1 ,2 ]
机构：
[1] RIKEN Cluster Pioneering Res, Synthet Organ Chem Lab, 2-1 Hirosawa, Wako, Saitama 3510198, Japan
[2] RIKEN Ctr Sustainable Resource Sci, 2-1 Hirosawa, Wako, Saitama 3510198, Japan
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2021年 / 19卷 / 38期
关键词：
DEUTERIUM-OXIDE; VISUALIZATION; ACID;
D O I：
10.1039/d1ob01479j
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Alkynes were employed as tags to observe small molecules in cells by Raman microscopy. Herein, simple deuteration was found to shift the vibrational frequency of the alkyne by 135 cm(-1). Two-color Raman imaging of D-alkynes and H-alkynes made it possible to distinguish between and observe similar small molecules in live cells.
引用
收藏
页码：8232 / 8236
页数：5
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