Organic Polymer Monolith: Synthesis and Applications For Bioanalytical

High-speed separation, enrichment, digestion, as well as high-throughput analysis of biomolecules are very important in analytical and bioanalytical chemistry, biosciences, etc. for thesepurposes, currently a single piece of porous material so-called "monolith", Has been rapidly developed for Several decadesand nowadays holds an impressively strong position in separation science and further extended in other areas of chemistry ie media extraction and catalyst supports, Recently, we are Involved in development of analytical procedures for separation and accurate detection of biomolecules through the development of organic polymer-based Monoliths coupled with chromatographic systems. The addition of monolith columns as the stationary phase HPLC on the system has been proven to improve the performance of the system, since monolith possesses a number of advantages over conventional packed-column. Uniformity of bed with no end frits, higher permeability, and fast separation / reaction time are the main advantages of monolithic stationary phase. Reviews These Monoliths, the which were coupled into HPLC as well as CFFN HPLC-ICP / MS system, were applied to separation and Quantificationof ssDNA, dsDNA, methylated DNA, single nucleotide polymorphism (SNP), protein, peptide mapping, and enrichment of Phosphopeptide. So far, we have Monoliths prepared a reverse phase of poly (lauryl methacrylate-co-ethylene dimethacrylate)(Poly (LMA-co-EDMA)) inside microbore column. This monolith Allows rapid separation of common proteins, Also we successfully preparedanion exchange monoliths poly (GMA-co-EDMA) modified with Diethylamine inside 1.0 mm id and 0.05mm. id columns for the separation of DNA samples, Methylated DNA, and SNP, Monoliths have great potential as highly efficient catalyst support. For this purpose, trypsin is then Introduced to the monolithic support for high speed protein digestions. As catalyst support, monolith Also facilitates the flow of organic synthesis. Poly (GMA-co-EDMA) -based Monoliths modified with ligands of 5-amino-1,10-Phenanthroline and chemical immobilization of Pd was used for flow-through Suzuki-Miyaura cross-coupling reaction, Enrichment of phosphopeptides from the digest of the protein mixture is conducted using immobilized metal ion (Ti4 +) affinity chromatography (IMAC) monolith.