Characterization of Human Anti-Dengue NS1 Monoclonal Antibodies Derived from Patients Infected with DENV-2

被引：0
|
作者：
Kowaboot, Siriporn [1 ]
Puangmanee, Wilarat [1 ]
Benjathummarak, Surachet [2 ]
Boonha, Khwanchit [2 ]
Chaisri, Urai [3 ]
Ramasoota, Pongrama [1 ,2 ]
Pitaksajjakul, Pannamthip [1 ,2 ]
机构：
[1] Mahidol Univ, Fac Trop Med, Dept Social & Environm Med, Bangkok, Thailand
[2] Mahidol Univ, Fac Trop Med, Ctr Excellence Antibody Res, Bangkok, Thailand
[3] Mahidol Univ, Fac Trop Med, Dept Trop Pathol, Bangkok, Thailand
来源：
JAPANESE JOURNAL OF INFECTIOUS DISEASES | 2022年 / 75卷 / 01期
关键词：
NONSTRUCTURAL PROTEIN-1; CROSS-REACT; VIRUS; PATHOGENESIS; PLASMINOGEN;
D O I：
10.7883/yoken.JJID.2020.1071
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Mouse antibodies specific to dengue NS1 have been widely investigated for their cross-reactivity with several human biomolecules. This is the first study demonstrating the cross-reactivity of human monoclonal antibodies (HuMAbs) specific to dengue NS1 isolated from patients infected with dengue virus serotype-2 (DENV-2). Nine anti-NS1 HuMAbs, which were mainly derived from patients in convalescent-phase after secondary infection of DENV-2, were characterized. Their cross-reactivity with plasminogen, thrombin, and endothelial cells was investigated, following which plasmin-formation assays were performed. All anti-NS1 HuMAbs exhibited cross-reactivity with human plasminogen (Pig), but not with thrombin or endothelial cells. Moreover, all HuMAbs exhibiting cross-reactivity with Plg converted Plg to plasmin in the plasmin-formation assay. These results suggest the implications and drawbacks of using anti-NS1 antibodies in immunotherapy.
引用
收藏
页码：24 / 30
页数：7
相关论文
共 50 条
  • [1] Production of anti-dengue NS1 monoclonal antibodies by DNA immunization
    Puttikhunt, C
    Kasinrerk, W
    Srisa-ad, S
    Duangchinda, T
    Silakate, W
    Moonsom, S
    Sittisombut, N
    Malasit, P
    [J]. JOURNAL OF VIROLOGICAL METHODS, 2003, 109 (01) : 55 - 61
  • [2] Selection and Characterization of Anti-Dengue NS1 Single Domain Antibodies
    Lisa C. Shriver-Lake
    Jinny L. Liu
    Dan Zabetakis
    Victor A. Sugiharto
    Cheng-Rei Lee
    Gabriel N. Defang
    Shuenn-Jue L. Wu
    George P. Anderson
    Ellen R. Goldman
    [J]. Scientific Reports, 8
  • [3] Characterization of two anti-dengue human monoclonal antibodies prepared from PBMCs of patients with dengue illness in Thailand
    Li, Z. -Y.
    Yamashita, A.
    Kawashita, N.
    Sasaki, T.
    Pan, Y.
    Ono, K. -I.
    Ikuta, K.
    Li, Y. -G.
    [J]. ACTA VIROLOGICA, 2016, 60 (02) : 166 - 173
  • [4] Nanodelivery system enhances the immunogenicity of dengue-2 nonstructural protein 1, DENV-2 NS1
    Jearanaiwitayakul, Tuksin
    Sunintaboon, Panya
    Chawengkittikul, Runglawan
    Limthongkul, Jitra
    Midoeng, Panuwat
    Warit, Saradee
    Ubol, Sukathida
    [J]. VACCINE, 2020, 38 (43) : 6814 - 6825
  • [5] IDENTIFICATION AND CHARACTERIZATION OF NOVEL HUMAN ANTI-DENV-2 MONOCLONAL ANTIBODIES THAT DO NOT TARGET DOMAIN III OF THE DENV-2 ENVELOPE GLYCOPROTEIN
    Messer, William
    de Alwis, Ruklanthi
    Quang Pham
    Jeremy Huynh
    Betramello, Martina
    Sallustro, Federica
    Lanzavecchia, Antonio
    Baric, Ralph S.
    de Silva, Aravinda
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2010, 83 (05): : 141 - 141
  • [6] STUDY OF ANTI-DENGUE NS1 ANTIBODY BY WESTERN-BLOT
    KUNO, G
    VORNDAM, AV
    GUBLER, DJ
    GOMEZ, I
    [J]. JOURNAL OF MEDICAL VIROLOGY, 1990, 32 (02) : 102 - 108
  • [7] Development of an anti-dengue NS1 IgG ELISA to evaluate exposure to dengue virus
    Nascimento, Eduardo J. M.
    George, James K.
    Velasco, Melissa
    Bonaparte, Matthew I.
    Zheng, Lingyi
    DiazGranados, Carlos A.
    Marques, Ernesto T. A.
    Huleatt, James W.
    [J]. JOURNAL OF VIROLOGICAL METHODS, 2018, 257 : 48 - 57
  • [8] Selection and Characterization of Anti- Dengue NS1 Single Domain Antibodies
    Shriver-Lake, Lisa C.
    Liu, Jinny L.
    Zabetakis, Dan
    Sugiharto, Victor A.
    Lee, Cheng-Rei
    Defang, Gabriel N.
    Wu, Shuenn-Jue L.
    Anderson, George P.
    Goldman, Ellen R.
    [J]. SCIENTIFIC REPORTS, 2018, 8
  • [9] Investigating Lycotoxin-An1a (An1a), a defense antiviral peptide from Alopecosa nagpag venom as prospective anti-dengue agent against DENV-2 NS2B-NS3 protease
    Purohit, Priyanka
    Barik, Debashis
    Dansana, Jarmani
    Meher, Biswa Ranjan
    [J]. COMPUTATIONAL BIOLOGY AND CHEMISTRY, 2024, 108
  • [10] Clinical performance evaluation of VIDAS® dengue NS1 Ag, VIDAS® Anti-DENGUE IgM and VIDAS® Anti-DENGUE IgG assays in endemic population
    Tessonneau, M.
    Foussadier, A.
    Dromenq, L.
    Brossault, L.
    [J]. TROPICAL MEDICINE & INTERNATIONAL HEALTH, 2021, 26 : 99 - 100
12345