Rapid screening of marine bacterial symbionts using MALDI-TOF MS

被引：11
|
作者：
Vidal, Livia M. R. [1 ]
Venas, Taina M. [1 ]
Goncalves, Aline R. P. [1 ]
Mattsson, Hannah K. [1 ]
Silva, Raphael V. P. [1 ]
Nobrega, Maria S. [1 ]
Azevedo, Gustavo P. R. [1 ]
Garcia, Gizele D. [1 ,2 ]
Tschoeke, Diogo A. [1 ,3 ]
Vieira, Veronica V. [4 ]
Thompson, Fabiano L. [1 ]
Thompson, Cristiane C. [1 ]
机构：
[1] Fed Univ Rio de Janeiro UFRJ, Inst Biol, Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro UFRJ, Dept Ensino Grad, Campus UFRJ Macae Prof Aloisio Teixeira, Macae, RJ, Brazil
[3] Univ Fed Rio de Janeiro, COPPE, Biomed Engn Program, Rio de Janeiro, Brazil
[4] Fiocruz MS, Oswaldo Cruz Fdn, Interdisciplinary Med Res Lab, Rio De Janeiro, Brazil
来源：
ARCHIVES OF MICROBIOLOGY | 2020年 / 202卷 / 08期
关键词：
MALDI-TOF; Screening tool; Marine bacterial symbionts; Vibrio; pyrHgene; DESORPTION-IONIZATION-TIME; SAMPLE PREPARATION METHODS; MASS-SPECTROMETRY; IDENTIFICATION; DIVERSITY; DIFFERENTIATION; REPRODUCIBILITY; CLASSIFICATION; SEAWATER; VIBRIOS;
D O I：
10.1007/s00203-020-01917-9
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) is a rapid, cost-effective and high-throughput method for bacteria characterization. However, most previous studies focused on clinical isolates. In this study, we evaluated the use of MALDI-TOF MS as a rapid screening tool for marine bacterial symbionts. A set of 255 isolates from different marine sources (corals, sponge, fish and seawater) was analyzed using cell lysates to obtain a rapid grouping. Cluster analysis of mass spectra and 16S rRNA showed 18 groups, includingVibrio, Bacillus, Pseudovibrio, AlteromonasandRuegeria. MALDI-TOF distance similarity scores >= 60% and >= 70% correspond to >= 98.7% 16S rRNA gene sequence similarity and >= 95%pyrHgene sequence similarity, respectively. MALDI-TOF MS is a useful tool forVibriospecies groups' identification.
引用
收藏
页码：2329 / 2336
页数：8
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