EM703, a new derivative of erythromycin, inhibits transforming growth factor-β signaling in human lung fibroblasts

被引：11
|
作者：
Yu, ChangHe [1 ]
Azuma, Arata [1 ]
Li, YingJi [2 ,3 ]
Wang, Chunyan [1 ]
Abe, Sinji [1 ]
Usuki, Jiro [1 ]
Matsuda, Kuniko [1 ]
Kudoh, Shoji [1 ]
机构：
[1] Nippon Med Sch, Dept Internal Med Pulm Med Infect & Oncol, Tokyo 1138602, Japan
[2] Nippon Med Sch, Dept Hyg, Tokyo 1138602, Japan
[3] Nippon Med Sch, Dept Publ Hlth, Tokyo 1138602, Japan
来源：
EXPERIMENTAL LUNG RESEARCH | 2008年 / 34卷 / 06期
关键词：
EM703; fibroblast; IPF; macrolides; Smad; TGF-beta;
D O I：
10.1080/01902140802093238
中图分类号：
R56 [呼吸系及胸部疾病];
学科分类号：
摘要：
Long-term, low-dose macrolide therapy has been proven to improve survival in patients with diffuse panbronchiolitis and cystic fibrosis, although the mechanisms by which it does so remain unknown. To elucidate the molecular mechanisms of the anti-inflammatory effects of macrolides, the authors examined the effects of erythromycin (EM-A) and new derivative EM703 on transforming growth factor (TGF)-beta/Smad signaling fibroblasts. EM-A and EM703 each inhibited fibroblast proliferation and the collagen production in human lung fibroblasts induced by TGF-beta. EM-A and EM703 inhibited the augmentation of Smad3 mRNA induced by TGF-beta. Smad7 mRNA was inhibited by TGF-beta, but augmented by coincubation with EM-A or EM703. EM-A and EM703 each inhibited p-Smad2/3 proteins induced by TGF-beta. Smad7 protein inhibited by TGF-beta restored beyond basal level by EM-A and EM703. These findings suggest that EM-A and EM703 inhibit TGF-beta signaling in human lung fibroblasts via inhibition of p-Smad2/3 through recovery of Smad7 level.
引用
收藏
页码：343 / 354
页数：12
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