Transplantation of Bone Marrow-Derived Mesenchymal Stem Cells into the Developing Mouse Eye

被引:12
|
作者
Lee, Eun-Shil [2 ]
Yu, Song-Hee [2 ]
Jang, Yu-Jin [2 ]
Hwang, Dong-Youn [3 ]
Jeon, Chang-Jin [1 ,2 ]
机构
[1] Kyungpook Natl Univ, Neurosci Lab, Dept Biol, Coll Nat Sci, Taegu 702701, South Korea
[2] Kyungpook Natl Univ, Brain Sci & Engn Inst, Taegu 702701, South Korea
[3] CHA Univ, Dept Biomed Sci, Coll Life Sci, Seoul 13581, South Korea
关键词
bone marrow-derived mesenchymal stem cells; retina; transplantation; differentiation; integration; NEURAL PROGENITOR CELLS; STROMAL CELLS; NEUROTROPHIC FACTOR; ADULT-RAT; MORPHOLOGICAL INTEGRATION; FATE DETERMINATION; MULLER GLIA; DIFFERENTIATION; RETINA; EXPRESSION;
D O I
10.1267/ahc.11009
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Mesenchymal stem cells (MSCs) have been studied widely for their potential to differentiate into various lineage cells including neural cells in vitro and in vivo. To investigate the influence of the developing host environment on the integration and morphological and molecular differentiation of MSCs, human bone marrow-derived mesenchymal stem cells (BM-MSCs) were transplanted into the developing mouse retina. Enhanced green fluorescent protein (GFP)-expressing BM-MSCs were transplanted by intraocular injections into mice, ranging in ages from 1 day postnatal (PN) to 10 days PN. The survival dates ranged from 7 days post-transplantation (DPT) to 28DPT, at which time an immunohistochemical analysis was performed on the eyes. The transplanted BM-MSCs survived and showed morphological differentiation into neural cells and some processes within the host retina. Some transplanted cells expressed microtubule associated protein 2 (MAP2ab, marker for mature neural cells) or glial fibrillary acid protein (GFAP, marker for glial cells) at 5PN 7DPT. In addition, some transplanted cells integrated into the developing retina. The morphological and molecular differentiation and integration within the 5PN 7DPT eye was greater than those of other-aged host eye. The present findings suggest that the age of the host environment can strongly influence the differentiation and integration of BM-MSCs.
引用
收藏
页码:213 / 221
页数:9
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