In vitro comparison of cryopreserved and liquid platelets: potential clinical implications
被引:56
|
作者:
Johnson, Lacey
论文数: 0引用数: 0
h-index: 0
机构:
Australian Red Cross Blood Serv, Res & Dev, Sydney, NSW, AustraliaAustralian Red Cross Blood Serv, Res & Dev, Sydney, NSW, Australia
Johnson, Lacey
[1
]
Reade, Michael C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Queensland, Burns Trauma & Crit Care Res Ctr, Brisbane, Qld, Australia
Australian Def Force, Joint Hlth Command, Canberra, ACT, AustraliaAustralian Red Cross Blood Serv, Res & Dev, Sydney, NSW, Australia
Reade, Michael C.
[2
,3
]
Hyland, Ryan A.
论文数: 0引用数: 0
h-index: 0
机构:
Australian Red Cross Blood Serv, Res & Dev, Sydney, NSW, AustraliaAustralian Red Cross Blood Serv, Res & Dev, Sydney, NSW, Australia
Hyland, Ryan A.
[1
]
Tan, Shereen
论文数: 0引用数: 0
h-index: 0
机构:
Australian Red Cross Blood Serv, Res & Dev, Sydney, NSW, AustraliaAustralian Red Cross Blood Serv, Res & Dev, Sydney, NSW, Australia
Tan, Shereen
[1
]
Marks, Denese C.
论文数: 0引用数: 0
h-index: 0
机构:
Australian Red Cross Blood Serv, Res & Dev, Sydney, NSW, AustraliaAustralian Red Cross Blood Serv, Res & Dev, Sydney, NSW, Australia
Marks, Denese C.
[1
]
机构:
[1] Australian Red Cross Blood Serv, Res & Dev, Sydney, NSW, Australia
[2] Univ Queensland, Burns Trauma & Crit Care Res Ctr, Brisbane, Qld, Australia
[3] Australian Def Force, Joint Hlth Command, Canberra, ACT, Australia
BackgroundPlatelet (PLT) concentrates can be cryopreserved in dimethyl sulfoxide (DMSO) and stored at -80 degrees C for 2 years. These storage conditions improve availability in both rural and military environments. Previous phenotypic and in vitro studies of cryopreserved PLTs are limited by comparison to fresh liquid-stored PLTs, rather than PLTs stored over their clinically relevant shelf life. Further, nothing is known of the effect of reconstituting cryopreserved PLTs in plasma stored at a variety of clinically relevant temperatures. Study Design and MethodsApheresis PLTs were either stored at room temperature for 5 days or cryopreserved at -80 degrees C with 5% DMSO. Cryopreserved PLTs were thawed at 37 degrees C and reconstituted in plasma (stored at different temperatures) and compared to fresh and expired liquid-stored PLTs. In vitro assays were performed to assess glycoprotein expression, PLT activity, microparticle content, and function. ResultsCompared to liquid PLTs over storage, cryopreserved PLTs had reduced expression of the key glycoprotein receptors GPIb and GPIIb. However, the proportion of PLTs expressing activation markers CD62P and CD63 was similar between cryopreserved and liquid-stored PLTs at expiry. Cryopreserved PLT components contained significantly higher numbers of phosphatidylserine- and tissue factor-positive microparticles than liquid-stored PLTs, and these microparticles reduced the time to clot formation and increased thrombin generation. ConclusionThere are distinct differences between cryopreserved and liquid-stored PLTs. Cryopreserved PLTs also have an enhanced hemostatic activity. Knowledge of these in vitro differences will be essential to understanding the outcomes of a clinical trial comparing cryopreserved PLTs and liquid PLTs stored for various durations.
机构:
Hosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, BrazilHosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, Brazil
Bub, C. B.
Yohoyama, A.
论文数: 0引用数: 0
h-index: 0
机构:
Hosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, BrazilHosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, Brazil
Yohoyama, A.
Conti, F. M.
论文数: 0引用数: 0
h-index: 0
机构:
Hosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, BrazilHosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, Brazil
Conti, F. M.
de Paula, T. A.
论文数: 0引用数: 0
h-index: 0
机构:
Hosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, BrazilHosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, Brazil
de Paula, T. A.
Costa, T. H.
论文数: 0引用数: 0
h-index: 0
机构:
Hosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, BrazilHosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, Brazil
Costa, T. H.
de Almeida, L. F.
论文数: 0引用数: 0
h-index: 0
机构:
Hosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, BrazilHosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, Brazil
de Almeida, L. F.
Sirianni, M. F.
论文数: 0引用数: 0
h-index: 0
机构:
Hosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, BrazilHosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, Brazil
Sirianni, M. F.
Lima, J. K.
论文数: 0引用数: 0
h-index: 0
机构:
Hosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, BrazilHosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, Brazil
Lima, J. K.
Aravechia, M. G.
论文数: 0引用数: 0
h-index: 0
机构:
Hosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, BrazilHosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, Brazil
Aravechia, M. G.
Kutner, J. M.
论文数: 0引用数: 0
h-index: 0
机构:
Hosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, BrazilHosp Israelita Albert Einstein, Hemotherapy & Cell Therapy Dept, Sao Paulo, Brazil