On-Chip Screening of a Glycomimetic Library with C-Type Lectins Reveals Structural Features Responsible for Preferential Binding of Dectin-2 over DC-SIGN/R and Langerin

被引：14

作者：

Medve, Laura ^{[1
]}

Achilli, Silvia ^{[2
]}

Serna, Sonia ^{[3
]}

Zuccotto, Fabio ^{[4
]}

Varga, Norbert ^{[1
]}

Thepaut, Michel ^{[2
]}

Civera, Monica ^{[1
]}

Vives, Corinne ^{[2
]}

Fieschi, Franck ^{[2
]}

Reichardt, Niels ^{[3
,5
]}

Bernardi, Anna ^{[1
]}

机构：

[1] Univ Milan, Dipartimento Chim, I-20133 Milan, Italy

[2] Univ Grenoble Alpes, CNRS, CEA, Inst Biol Struct, F-38000 Grenoble, France

[3] CIC BiomaGUNE, Glycotechnol Lab, Paseo Miramon 182, Donostia San Sebastian 20014, Spain

[4] Univ Dundee, Dundee, Scotland

[5] CIBER, BBN, Donostia San Sebastian 20074, Spain

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2018年 / 24卷 / 54期

关键词：

carbohydrates; C-type lectins; drug discovery; glycomimetics; microarrays; MEDIATED HIV-INFECTION; CARBOHYDRATE-PROTEIN INTERACTIONS; PATTERN-RECOGNITION RECEPTOR; GLYCAN MICROARRAYS; DENDRITIC CELLS; CUTTING EDGE; HIGH-MANNOSE; T-CELLS; AFFINITY; DESIGN;

D O I：

10.1002/chem.201802577

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A library of mannose- and fucose-based glycomimetics was synthesized and screened in a microarray format against a set of C-type lectin receptors (CLRs) that included DC-SIGN, DC-SIGNR, langerin, and dectin-2. Glycomimetic ligands able to interact with dectin-2 were identified for the first time. Comparative analysis of binding profiles allowed their selectivity against other CLRs to be probed.

引用

页码：14448 / 14460

页数：13