Isolation of microRNA targets using biotinylated synthetic microRNAs

被引：134
|
作者：
Orom, Ulf Andersson
Lund, Anders H.
机构：
[1] Biotech Res & Innovat Ctr, DK-2200 Copenhagen, Denmark
[2] Univ Copenhagen, Fac Hlth Sci, DK-2200 Copenhagen, Denmark
来源：
METHODS | 2007年 / 43卷 / 02期
关键词：
MicroRNA; MicroRNA target isolation; MicroRNA target identification;
D O I：
10.1016/j.ymeth.2007.04.007
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
MicroRNAs are small regulatory RNAs found in multicellular organisms where they post-transcriptionally regulate gene expression. In animals, microRNAs bind mRNAs via incomplete base pairings making the identification of microRNA targets inherently difficult. Here, we present a detailed method for experimental identification of microRNA targets based on affinity purification of tagged microRNAs associated with their targets. (C) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：162 / 165
页数：4
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