New cytotoxic flavonoids from aerial parts of Platycladus orientalis L.

Investigation ofPlatycladus orientalisyielded five flavonoids, including aglycone flavone1(apigenin), flavone glycoside2(apigenin 7-O-beta-D-glucopyranoside), new gernaylated flavone glycoside3(apigenin 8-gernayl-4 '-O-alpha-gluco pyranoside) and two new pernylated flavonoid glycosides4&5(apigenin 8-pernyl-4 '-glucopyranosyl-7-O-alpha-glucopyranoside and apigenin 5-pernyl-7-glucopyranosyl-4 '-O-beta-D-glucopyranoside). Their structures were elucidated on the basis of spectroscopic evidence. The cytotoxicity of compounds1-5were tested against Lung adenocarcinoma (A549), human hepatocellular liver carcinoma (HepG2), human breast carcinoma (MCF-7) cell lines and mouse fibroblast cell line NIH/3T3 as normal cells. This assay gave spot on structure activity relationship which, showed that cytotoxicity of compounds (1)and (2)against three cell lines was weak as IC50 > 15. Compounds(4)and (5)had moderate cytotoxic and no toxic effect on normal cell. Compound(3) showed high cytotoxic activity against tested three cell lines with no toxic effect of normal cells.