Transporting behavior of valinomycin carrying Rb+ across fluid membrane into human erythrocyte

被引：0

作者：

Wang Ya-Ni ^{[1
]}

Jiang Yu-Cheng ^{[1
]}

Feng Yun-Xiao ^{[1
]}

Hu Man-Cheng ^{[1
]}

Li Shu-Ni ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Sch Chem & Mat Sci, Key Lab Macromol Sci Shaanxi Prov, Xian 710062, Peoples R China

来源：

ACTA CHIMICA SINICA | 2008年 / 66卷 / 13期

关键词：

Rb+; transmembrane behavior; valinomycin; graphite furnace atomic absorption spectrometry; fluorescence;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Transmembrane behavior of valinomycin carrying Rb+ across an erythrocyte membrane was studied through the measurements of Rb+ content in cell by graphite furnace atomic absorption spectrometry. The influence of several parameters, such as transporting rate and uptake of Rb+ by the erythrocyte was studied based on the dynamics investigation of the transmembrane process. In addition, the effect of RbCl on the fluorescence intensity of the erythrocyte membrane protein was also examined in this work. The results indicate that the action of valinomycin carrying Rb+ transporting into the erythrocyte has a kinetic characteristics of series stair reaction. The uptake of Rb+ by the erythrocyte is mainly controlled by ligand concentration and incubation time. Only high concentration of RbCl can affect the fluorescence intensity of the membrane protein.

引用

页码：1541 / 1545

页数：5

共 24 条

[1] VALINOMYCIN-INDUCED UPTAKE OF POTASSIUM IN MEMBRANE VESICLES FROM ESCHERICHIA-COLI - (K-UPTAKE MUTANTS/ACTIVE TRANSPORT)
BHATTACHARYYA, P
EPSTEIN, W
SILVER, S
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1971, 68 (07) : 1488 - +
[2] CHANG R, 1986, PHYS CHEM APPL BIOL, P303
[3] PREPARATION AND CHEMICAL CHARACTERISTICS OF HEMOGLOBIN-FREE GHOSTS OF HUMAN ERYTHROCYTES
DODGE, JT
HANAHAN, DJ
MITCHELL, C
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1963, 100 (01) : 119 - &
[4] Feng YX, 2007, CHINESE J INORG CHEM, V23, P421
[5] Gao YX, 1999, ACTA CHIM SINICA, V57, P659
[6] Volumetric properties and volumetric interaction parameters for the CsCl-monosaccharide (D-galactose, D-xylose and D-arabinose)-water systems at T=298.15 K
Jiang, YC
Hu, MC
Wang, JJ
Zhuo, KL
Xia, SP
[J]. JOURNAL OF CHEMICAL THERMODYNAMICS, 2004, 36 (08): : 671 - 676
[7] Jiang YC, 2003, CHEM J CHINESE U, V24, P1842
[8] JIANG YC, 2002, RARE METALS, V26, P299
[9] JOHN L, 2000, Patent No. 6011061
[10] LOWRY OH, 1951, J BIOL CHEM, V193, P265

← 1 2 3 →