Hybrid Methods in Iron-Sulfur Cluster Biogenesis

被引:6
|
作者
Prischi, Filippo [1 ]
Pastore, Annalisa [2 ,3 ]
机构
[1] Univ Essex, Sch Biol Sci, Colchester, Essex, England
[2] King s Coll London, Maurice Wohl Inst, London, England
[3] Univ Pavia, Mol Med Dept, Pavia, Italy
基金
英国生物技术与生命科学研究理事会;
关键词
frataxin; NMR; molecular complexes; small angle X-ray scattering; structural biology; iron-sulfur cluster machinery; hybrid methods; ANGLE X-RAY; NUCLEAR-MAGNETIC-RESONANCE; STRUCTURAL-CHARACTERIZATION; SOLUTION SCATTERING; BACTERIAL FRATAXIN; BIOLOGICAL MACROMOLECULES; CYSTEINE DESULFURASE; CRYSTAL-STRUCTURE; GENE-CLUSTER; COMBINED NMR;
D O I
10.3389/fmolb.2017.00012
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hybrid methods, which combine and integrate several biochemical and biophysical techniques, have rapidly caught up in the last twenty years to provide a way to obtain a fuller description of proteins and molecular complexes with sizes and complexity otherwise not easily affordable. Here, we review the use of a robust hybrid methodology based on a mixture of NMR, SAXS, site directed mutagenesis and molecular docking which we have developed to determine the structure of weakly interacting molecular complexes. We applied this technique to gain insights into the structure of complexes formed amongst proteins involved in the molecular machine, which produces the essential iron-sulfur cluster prosthetic groups. Our results were validated both by X-ray structures and by other groups who adopted the same approach. We discuss the advantages and the limitations of our methodology and propose new avenues, which could improve it.
引用
收藏
页数:13
相关论文
共 50 条
  • [1] Iron-Sulfur Cluster Biogenesis and Iron Homeostasis
    Fontenot, Chelsey
    Tasnim, Homyra
    Ding, Huangen
    [J]. FREE RADICAL BIOLOGY AND MEDICINE, 2019, 145 : S23 - S23
  • [2] Iron-Sulfur Cluster Biogenesis and Iron Homeostasis in Cyanobacteria
    Gao, Fudan
    [J]. FRONTIERS IN MICROBIOLOGY, 2020, 11
  • [3] Iron-Sulfur Cluster Biogenesis Systems and their Crosstalk
    Xu, Xiang Ming
    Moller, Simon G.
    [J]. CHEMBIOCHEM, 2008, 9 (15) : 2355 - 2362
  • [4] Iron-sulfur cluster biogenesis and trafficking in mitochondria
    Braymer, Joseph J.
    Lill, Roland
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2017, 292 (31) : 12754 - 12763
  • [5] The role of chaperones in iron-sulfur cluster biogenesis
    Puglisi, Rita
    Pastore, Annalisa
    [J]. FEBS LETTERS, 2018, 592 (24): : 4011 - 4019
  • [6] Iron-sulfur cluster biogenesis and human disease
    Rouault, Tracey A.
    Tong, Wing Hang
    [J]. TRENDS IN GENETICS, 2008, 24 (08) : 398 - 407
  • [7] Iron-Sulfur Cluster Biogenesis as a Critical Target in Cancer
    Petronek, Michael S.
    Spitz, Douglas R.
    Allen, Bryan G.
    [J]. ANTIOXIDANTS, 2021, 10 (09)
  • [8] Mitochondrial iron-sulfur cluster biogenesis and neurological disorders
    Selvanathan, Arthavan
    Sankaran, Bindu Parayil
    [J]. MITOCHONDRION, 2022, 62 : 41 - 49
  • [9] GTP is required for iron-sulfur cluster biogenesis in mitochondria
    Amutha, Boominathan
    Gordon, Donna M.
    Gu, Yajuan
    Lyver, Elise R.
    Dancis, Andrew
    Pain, Debkumar
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (03) : 1362 - 1371
  • [10] Structural Basis of RICs Iron Donation for Iron-Sulfur Cluster Biogenesis
    Silva, Liliana S. O.
    Matias, Pedro M.
    Romao, Celia V.
    Saraiva, Ligia M.
    [J]. FRONTIERS IN MICROBIOLOGY, 2021, 12