Decreased peroxidase activity in transgenic tobacco and its effect on lignification

被引：12
|
作者：
Talas-Ogras, T [1 ]
Kazan, K
Gözükirmizi, N
机构：
[1] TUBITAK, Marmara Res Ctr, Res Inst Genet Engn & Biotechnol, TR-41470 Gebze, Kocaeli, Turkey
[2] Univ Queensland, Cooperat Res Ctr Trop Plant Pathol, Brisbane, Qld 4072, Australia
[3] Univ Istanbul, Fac Sci, Dept Biol, TR-34459 Istanbul, Turkey
来源：
BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 04期
关键词：
antisense expression; lignification; peroxidase;
D O I：
10.1023/A:1005602213270
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An antisense gene construct of a peroxidase gene (Shpx6a) from a tropical pasture legume Stylosanthes humilis was transferred into tobacco cells via Agrobacterium tumefaciens to test whether peroxidase activity could be decreased and what effect this would have on lignification. A large number of tobacco cell lines were regenerated on selective media and stable integration of the transgene was confirmed in randomly selected putative transformants. Analyses of the primary transgenic plants and their progeny (T-1) demonstrated that the total peroxidase activity was significantly decreased (up to 36%) as compared to that measured in untransformed control plants. Importantly, reduction in peroxidase activity is accompanied by decreases (up to 23%) in lignin content in several transgenic lines.
引用
收藏
页码：267 / 273
页数：7
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