Transcription activation by a variety of AraC/XylS family activators does not depend on the class II-specific activation determinant in the N-terminal domain of the RNA polymerase alpha subunit

被引：17

作者：

Egan, SM ^{[1
]}

Pease, AJ

Lang, J

Li, X

Rao, V

Gillette, WK

Ruiz, R

Ramos, JL

Wolf, RE

机构：

[1] Univ Kansas, Dept Mol Biosci, Lawrence, KS 66045 USA

[2] Univ Maryland Baltimore Cty, Dept Biol Sci, Baltimore, MD 21250 USA

[3] NIDDKD, Mol Biol Lab, NIH, Bethesda, MD 20892 USA

[4] Dept Plant Biochem & Mol & Cellular Biol Plants, CSIC, Estac Expt Zaidin, Granada, Spain

来源：

JOURNAL OF BACTERIOLOGY | 2000年 / 182卷 / 24期

关键词：

D O I：

10.1128/JB.182.24.7075-7077.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The N-terminal domain of the RNA polymerase or subunit (alpha -NTD) was tested for a role in transcription activation by a variety of AraC/XylS family members. Based on substitutions at residues 162 to 165 and an extensive genetic screen we conclude that alpha -NTD is not an activation target for these activators.

引用

页码：7075 / 7077

页数：3

共 7 条

[1] Transcription Activation by Escherichia coli Rob at Class II Promoters: Protein-Protein Interactions between Rob's N-Terminal Domain and the σ70 Subunit of RNA Polymerase
Taliaferro, Lanyn P.
Keen, Edward F., III
Sanchez-Alberola, Neus
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JOURNAL OF MOLECULAR BIOLOGY, 2012, 419 (3-4) : 139 - 157
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EMBO JOURNAL, 1998, 17 (12): : 3439 - 3447
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BURATOWSKI, S
SHARP, PA
MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (10) : 5562 - 5564
[4] Transcription activation at Class I FNR-dependent promoters: identification of the activating surface of FNR and the corresponding contact site in the C-terminal domain of the RNA polymerase alpha subunit
Williams, SM
Savery, NJ
Busby, SJW
Wing, HJ
NUCLEIC ACIDS RESEARCH, 1997, 25 (20) : 4028 - 4034
[5] Amino acid residues in the alpha-subunit C-terminal domain of Escherichia coli RNA polymerase involved in activation of transcription from the mtr promoter
Yang, J
Murakami, K
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Fujita, N
Ishihama, A
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JOURNAL OF BACTERIOLOGY, 1997, 179 (19) : 6187 - 6191
[6] Activation of bacteriophage Mu mom transcription by C protein does not require specific interaction with the carboxyl-terminal region of the α or σ70 subunit of Escherichia coli RNA polymerase
Sun, WY
Hattman, S
Fujita, N
Ishihama, A
JOURNAL OF BACTERIOLOGY, 1998, 180 (12) : 3257 - 3259
[7] C-TERMINAL DOMAIN (CTD) OF RNA-POLYMERASE-II AND N-TERMINAL SEGMENT OF THE HUMAN TATA-BINDING PROTEIN (TBP) CAN MEDIATE REMOTE AND PROXIMAL TRANSCRIPTIONAL ACTIVATION, RESPECTIVELY
SEIPEL, K
GEORGIEV, O
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SCHAFFNER, W
NUCLEIC ACIDS RESEARCH, 1993, 21 (24) : 5609 - 5615

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