共 2 条
Genome-wide comparison of Corynebacterium diphtheriae isolates from Australia identifies differences in the Pan-genomes between respiratory and cutaneous strains
被引:18
|作者:
Timms, Verlaine J.
[1
]
Nguyen, Trang
[2
,3
]
Crighton, Taryn
[2
,3
]
Yuen, Marion
[2
,3
]
Sintchenko, Vitali
[1
,2
,3
,4
]
机构:
[1] Westmead Hosp, Ctr Infect Dis & Microbiol, POB 533, Wentworthville, NSW 2145, Australia
[2] ICPMR Pathol West, Ctr Infect Dis, Sydney, NSW, Australia
[3] ICPMR Pathol West, Microbiol Lab Serv, Sydney, NSW, Australia
[4] Univ Sydney, Marie Bashir Inst Infect Dis & Biosecur, Sydney Med Sch, Sydney, NSW, Australia
来源:
关键词:
Whole genome sequencing;
Diphtheria;
Vaccine preventable disease;
Molecular epidemiology;
Pan-genome analysis;
Virulence;
INFECTION;
ENDOCARDITIS;
POPULATION;
DIVERSITY;
SEQUENCE;
D O I:
10.1186/s12864-018-5147-2
中图分类号:
Q81 [生物工程学(生物技术)];
Q93 [微生物学];
学科分类号:
071005 ;
0836 ;
090102 ;
100705 ;
摘要:
BackgroundCorynebacterium diphtheriae is the main etiological agent of diphtheria, a global disease causing life-threatening infections, particularly in infants and children. Vaccination with diphtheria toxoid protects against infection with potent toxin producing strains. However a growing number of apparently non-toxigenic but potentially invasive C. diphtheriae strains are identified in countries with low prevalence of diphtheria, raising key questions about genomic structures and population dynamics of the species. This study examined genomic diversity among 48 C. diphtheriae isolates collected in Australia over a 12-year period using whole genome sequencing. Phylogeny was determined using SNP-based mapping and genome wide analysis.ResultsC. diphtheriae sequence type (ST) 32, a non-toxigenic clone with evidence of enhanced virulence that has been also circulating in Europe, appears to be endemic in Australia. Isolates from temporospatially related patients displayed the same ST and similarity in their core genomes. The genome-wide analysis highlighted a role of pilins, adhesion factors and iron utilization in infections caused by non-toxigenic strains.ConclusionsThe genomic diversity of toxigenic and non-toxigenic strains of C. diphtheriae in Australia suggests multiple sources of infection and colonisation. Genomic surveillance of co-circulating toxigenic and non-toxigenic C. diphtheriae offer new insights into the evolution and virulence of pathogenic clones and can inform targeted public health actions and policy. The genomes presented in this investigation will contribute to the global surveillance of C. diphtheriae both for the monitoring of antibiotic resistance genes and virulent strains such as those belonging to ST32.
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