Landfill leachate treatment by graphite engineered anaerobic membrane bioreactor: Performance enhancement and membrane fouling mitigation

Low efficiency of anaerobic digestion and membrane fouling, treating landfill leachate, are big barriers in the application of anaerobic membrane bioreactor (AnMBR). Anaerobic digestion enhancement and membrane fouling mitigation of AnMBR with graphite addition, treating landfill leachate, were investigated in this study. The effect of graphite on organics removal, biogas production, methane content in biogas, membrane fouling, microbial responses and foulant compositions were analyzed. With the graphite addition, chemical oxygen de-mand (COD) removal of 78% was achieved for influent COD concentration of 3000 mg/l, which was significantly higher than the stage without graphite addition (65%) for influent COD concentration of 2000 mg/l. Similarly, methane content in biogas with graphite addition was 56%, while without graphite addition it was 46%. These digestion improvements were due to the promotion of organics degradation, facilitated by direct interspecies electron transfer (DIET) mechanism via graphite addition in AnMBR. The graphite addition prolonged membrane cleaning cycle from 13 days to 30 days. Protein content in loosely bound extracellular polymeric substance (LB-EPS) was the main fouling agent, which decreased with the graphite addition. The main mechanism behind membrane fouling mitigation was the protein content reduction in LB-EPS, which was biodegraded by Tricho-coccus being increased in relative abundance with the graphite addition. Furthermore, abundance of Deni-tratisoma decreased in anaerobic sludge and its accumulation reduced on membrane surface, subsequently membrane fouling was mitigated. Overall, graphite addition in AnMBR is a potential eco-innovative approach that efficiently removes pollutants from landfill leachate, enhances biogas quality and mitigates membrane fouling.