A novel technique with enhanced detection and quantitation of HPV-16 E1- and E2-mediated DNA replication

被引：36
|
作者：
Taylor, ER [1 ]
Morgan, IM [1 ]
机构：
[1] Univ Glasgow, Dept Vet Pathol, Inst Comparat Med, Glasgow G61 1QH, Lanark, Scotland
来源：
VIROLOGY | 2003年 / 315卷 / 01期
关键词：
HPV; replication; taqman;
D O I：
10.1016/S0042-6822(03)00588-9
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Transient DNA replication assays to detect papillomavirus E1/E2-mediated DNA replication have depended upon Southern blotting. This technique is hazardous (radioactive), labour intensive, semiquantitative, and physically limited in the number of samples that can be processed at any one time. We have overcome these problems by developing a real-time PCR protocol for the detection of E1/E2-mediated transient DNA replication. The results demonstrate detection of replication at levels not seen using Southern blotting demonstrating enhanced sensitivity. This technique is also, by definition, highly quantitative. Therefore, the real-time PCR technique is the optimal method for the detection of E1/E2-mediated DNA replication. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码：103 / 109
页数：7
相关论文
共 50 条
  • [1] The fidelity of HPV16 E1/E2-mediated DNA replication
    Taylor, ER
    Dornan, ES
    Boner, W
    Connolly, JA
    McNair, S
    Kannouche, P
    Lehmann, AR
    Morgan, IM
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (52) : 52223 - 52230
  • [2] Inhibition of HPV-16 E7 oncogenic activity by HPV-16 E2
    Gammoh, N.
    Isaacson, E.
    Tomaic, V.
    Jackson, D. J.
    Doorbar, J.
    Banks, L.
    ONCOGENE, 2009, 28 (23) : 2299 - 2304
  • [3] Inhibition of HPV-16 E7 oncogenic activity by HPV-16 E2
    N Gammoh
    E Isaacson
    V Tomaić
    D J Jackson
    J Doorbar
    L Banks
    Oncogene, 2009, 28 : 2299 - 2304
  • [4] Evidence Supporting a Role for TopBP1 and Brd4 in the Initiation but Not Continuation of Human Papillomavirus 16 E1/E2-Mediated DNA Replication
    Gauson, Elaine J.
    Donaldson, Mary M.
    Dornan, Edward S.
    Wang, Xu
    Bristol, Molly
    Bodily, Jason M.
    Morgan, Iain M.
    JOURNAL OF VIROLOGY, 2015, 89 (09) : 4980 - 4991
  • [5] Human papillomavirus 16 L2 inhibits the transcriptional activation function, but not the DNA replication function, of HPV-16 E2
    Okoye, A
    Cordano, P
    Taylor, ER
    Morgan, IM
    Everett, R
    Campo, MS
    VIRUS RESEARCH, 2005, 108 (1-2) : 1 - 14
  • [6] Molecular mechanism of HPV DNA replication initiation, mediated by E1 and E2 proteins
    Rana, Anshul
    Evande, Roxanne
    Biswas-Fiss, Esther
    Biswas, Subhasis
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2024, 300 (03) : S681 - S681
  • [7] HPV-16 E2 contributes to induction of HPV-16 late gene expression by inhibiting early polyadenylation
    Johansson, Cecilia
    Somberg, Monika
    Li, Xiaoze
    Winquist, Ellenor Backstrom
    Fay, Joanna
    Ryan, Fergus
    Pim, David
    Banks, Lawrence
    Schwartz, Stefan
    EMBO JOURNAL, 2012, 31 (14): : 3212 - 3227
  • [8] Replication of HCV in B and T cells, following E1/E2-mediated viral entry.
    Depla, E
    Ducatteeuw, A
    Mares, G
    Maertens, G
    HEPATOLOGY, 1997, 26 (04) : 706 - 706
  • [9] Effect of BPV1 E2-mediated inhibition of E6/E7 expression in HPV16-positive cervical carcinoma cells
    Moon, MS
    Lee, CJ
    Um, SJ
    Park, JS
    Yang, JM
    Hwang, ES
    GYNECOLOGIC ONCOLOGY, 2001, 80 (02) : 168 - 175
  • [10] Boosting with recombinant vaccinia increases HPV-16 E7-specific T cell precursor frequencies of HPV-16 E7-expressing DNA vaccines
    Chen, CH
    Wang, TL
    Hung, CF
    Pardoll, DM
    Wu, TC
    VACCINE, 2000, 18 (19) : 2015 - 2022
12345