Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice

被引:9
|
作者
Shirato, Kazuya [1 ]
Maejima, Madoka [1 ]
Hirai, Asuka [2 ]
Ami, Yasushi [2 ]
Takeyama, Natsumi [3 ]
Tsuchiya, Kotaro [3 ]
Kusanagi, Kouich [3 ]
Nunoya, Tetsuo [3 ]
Taguchi, Fumihiro [1 ,4 ]
机构
[1] Dept Virol III, Lab Acute Resp Viral Dis & Cytokines, Tokyo, Japan
[2] Natl Inst Infect Dis, Div Expt Anim Res, Tokyo 2080011, Japan
[3] Nippon Inst Biol Sci, Tokyo 1980024, Japan
[4] Nippon Vet & Life Sci Univ, Lab Virol & Viral Infect, Fac Vet Med, Tokyo 1808602, Japan
关键词
RESPIRATORY-SYNDROME CORONAVIRUS; MOUSE HEPATITIS-VIRUS; ANGIOTENSIN-CONVERTING ENZYME-2; FELINE AMINOPEPTIDASE N; FUNCTIONAL RECEPTOR; SARS CORONAVIRUS; EXPRESSION; SUSCEPTIBILITY; INFECTION; PROTEINS;
D O I
10.1007/s00705-010-0790-1
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Porcine epidemic diarrhea virus (PEDV) is the major causative agent of fatal diarrhea in piglets. To study the pathogenic features of PEDV using a mouse model, PEDV with virulence in mice is required. In pursuit of this, we adapted a tissue-culture-passed PEDV MK strain to suckling mouse brains. PEDV obtained after ten passages through the brains (MK-p10) had increased virulence for mice, and its fusion activity in cultured cells exceeded that of the original strain. However, the replication kinetics of MK and MK-p10 did not differ from each other in the brain and in cultured cells. The spike (S) protein of MK-p10 had four amino acid substitutions relative to the original strain. One of these (an H-to-R substitution at residue 1,381) was first detected in PEDV isolated after eight passages, and both this virus (MK-p8) and MK-p10 showed enhanced syncytium formation relative to the original MK strain and viruses isolated after two, four, and six passages, suggesting the possibility that the H-to-R mutation was responsible for this activity. This mutation could be also involved in the increased virulence of PEDV observed for MK-p10.
引用
收藏
页码:1989 / 1995
页数:7
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