Metabolic Engineering of Flavonoid Biosynthesis in Apple (Malus domestica Borkh.)

被引:0
|
作者
Szankowski, I. [1 ]
Li, H. [1 ]
Flachowsky, H. [2 ]
Hoefer, M. [2 ]
Hanke, M. -V. [2 ]
Fischer, T. [3 ]
Forkmann, G. [3 ]
Treutter, D. [4 ]
Schwab, W. [5 ]
Hoffmann, T. [5 ]
机构
[1] Leibniz Univ Hannover, Fruit Sci Sect, Inst Biol Prod Syst, Hannover, Germany
[2] Fed Ctr Breeding Res Cultivated Plants, Inst Fruit Breeding, Dresden, Germany
[3] Tech Univ Munich, Chair Ornamental Plants & Hort Plant Breeding, Munich, Germany
[4] Tech Univ Munich, Unit Fruit Sci, Munich, Germany
[5] Tech Univ Munich, Biomol Food Technol, Munich, Germany
关键词
anthocyanidin synthase; Lc transcription factor; RNAi; transgenic apple; UDP-galactose:flavonoid 3-O-galactosyltransferase; TRANSCRIPTION FACTOR; GENE; LC; EXPRESSION;
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Flavonoids are a large family of polyphenolic compounds with manifold functions in plants including pathogen defence. Present in a wide range of vegetables and fruits, flavonoids form an integral part of the human diet and confer multiple health benefits. Modifying flavonoid biosynthesis in fruit crops, such as apple, offers the opportunity to increase plant resistance against pathogens and the health benefit potential of the fruit. Both overexpression and RNAi-based suppression strategies were used to modify flavonoid biosynthesis in apple. Introducing the maize Lc transcription factor gene, responsible for controlling the expression of structural genes of the flavonoid biosynthetic pathway in maize, into Malus domestica Borkh. cv. 'Holsteiner Cox' resulted in increased mRNA levels for most of the structural genes of the flavonoid pathway. Lc transgenic plants accumulated higher levels of the anthocyanin idaein, the flavan-3-ols epicatechin, catechin, and some distinct dimeric proanthocyanidins. In a second approach, the consequences of RNAi silencing of the genes for anthocyanidin synthase (ANS) and UDPgalactose: flavonoid 3-O-galactosyltransferase (FGT) gene to induce a shift towards flavan-3-ols were examined. Preliminary results obtained from transgenic plants of the cv. 'Holsteiner Cox' and of the red-leaved type TNR 31-35 (a descendent from Malus sieversii var. sieversii f. niedzwetzkyana (Dieck)) revealed that suppression of ANS or FGT resulted in increased amounts of flavan-3-ols and flavonols. In the red leaved type TNR 31-35, anthocyanin accumulation decreased after ANS silencing.
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页码:511 / 516
页数:6
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