Effect of hyaluronan supplementation on boar sperm motility and membrane lipid architecture status after cryopreservation

被引：35
|
作者：
Peña, FJ
Johannisson, A
Wallgren, M
Rodriguez-Martinez, H
机构：
[1] Swedish Univ Agr Sci, Fac Vet Med, Dept Obstet & Gynecol, SE-75007 Uppsala, Sweden
[2] Univ Extremadura, Fac Vet Med, Dept Reprod & Obstet, Caceres 10071, Spain
[3] Swedish Univ Agr Sci, Fac Vet Med, Dept Anat & Histol, SE-75007 Uppsala, Sweden
[4] Qual Genet, Kavlinge, Sweden
来源：
THERIOGENOLOGY | 2004年 / 61卷 / 01期
关键词：
boar; sperm; cryopreservation; membrane lipid architecture; CASA; merocyanine-540;
D O I：
10.1016/S0093-691X(03)00181-X
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We investigated the effect of supplementing extended boar semen with different amounts of hyaluronan (HA) prior to freezing on post-thaw sperm characteristics. Using a split sample design, the effect of HA at a final concentration of 500 or 1000 mug/ml semen on post-thaw motility parameters, and membrane lipid architecture status assessed by merocyanine-540/YOPRO-1 and flow cytometry were evaluated. HA-supplementation improved motility parameters (P < 0.05 to P < 0.001) and decreased the percentage of hyperactivated spermatozoa (P < 0.05). HA-supplemented samples had more spermatozoa showing high lipid membrane stability as assessed with merocyanine-540. In conclusion, HA appeared to preserve post-thaw spermatozoa viability in vitro and maintained membrane stability after cryopreservation. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码：63 / 70
页数：8
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