The ssgB gene, encoding a member of the regulon of stress-response sigma factor σH, is essential for aerial mycelium septation in Streptomyces coelicolor A3(2)

被引：9

作者：

Sevcikova, B ^{[1
]}

Kormanec, J ^{[1
]}

机构：

[1] Slovak Acad Sci, Inst Mol Biol, Ctr Excellence Mol Med, Bratislava 84551, Slovakia

来源：

ARCHIVES OF MICROBIOLOGY | 2003年 / 180卷 / 05期

关键词：

differentiation; gene disruption; recombinant DNA; RNA polymerase; sigma factor; stress response; Streptomyces coelicolor A3(2);

D O I：

10.1007/s00203-003-0603-y

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Streptomyces coelicolor A3(2) gene ssgB belongs to the regulon of stress-response sigma factor sigma(H). By integrative transformation via double cross-over, a stable null mutant of ssgB was obtained. This mutation had no obvious effect on vegetative growth, but critically affected aerial mycelium septation. The S. coelicolor ssgB mutant produced aerial hyphae without any signs of septation into spore compartments. The mutation was complemented in trans by wild-type ssgB including the sigma(H)-dependent ssgBp promoter. The results proved that ssgB belongs a developmental branch of the sigma(H) regulon.

引用

页码：380 / 384

页数：5

共 25 条

[1] The ssgB gene, encoding a member of the regulon of stress-response sigma factor σH, is essential for aerial mycelium septation in Streptomyces coelicolor A3(2)
Beatrica Sevcikova
Jan Kormanec
Archives of Microbiology, 2003, 180 : 380 - 384
[2] Stress-response sigma factor σH is essential for morphological differentiation of Streptomyces coelicolor A3(2)
Sevciková, B
Benada, O
Kofronova, O
Kormanec, J
ARCHIVES OF MICROBIOLOGY, 2001, 177 (01) : 98 - 106
[3] Stress-response sigma factor σΗ is essential for morphological differentiation of Streptomyces coelicolor A3(2)
Beatrica Sevciková
Oldrich Benada
Olga Kofronova
Jan Kormanec
Archives of Microbiology, 2001, 177 : 98 - 106
[4] Stress-response sigma factor σH directs expression of the gltB gene encoding glutamate synthase in Streptomyces coelicolor A3(2)
Kormanec, J
Sevcikova, B
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2002, 1577 (01): : 149 - 154
[5] Identification and transcriptional characterization of the gene encoding the stress-response σ factor σH in Streptomyces coelicolor A3(2)
Kormanec, J
Sevcíková, B
Halgasová, N
Knirschová, R
Rezuchová, B
FEMS MICROBIOLOGY LETTERS, 2000, 189 (01) : 31 - 38
[6] The stress-response sigma factor σH controls the expression of ssgB, a homologue of the sporulation-specific cell division gene ssgA, in Streptomyces coelicolor A3(2)
Kormanec J.
Sevcikova B.
Molecular Genetics and Genomics, 2002, 267 (4) : 536 - 543
[7] The stress-response sigma factor σH controls the expression of ssgB, a homologue of the sporulation-specific cell division gene ssgA, in Streptomyces coelicolor A3(2)
Kormanec, J
Sevcikova, B
MOLECULAR GENETICS AND GENOMICS, 2002, 267 (04) : 536 - 543
[8] Activity of the Streptomyces coelicolor stress-response sigma factor σH is regulated by an anti-sigma factor
Sevcikova, B
Kormanec, J
FEMS MICROBIOLOGY LETTERS, 2002, 209 (02) : 229 - 235
[9] Cascade of sigma factors in streptomycetes:: identification of a new extracytoplasmic function sigma factor σJ that is under the control of the stress-response sigma factor σH in Streptomyces coelicolor A3(2)
Mazurakova, Vladislava
Sevcikova, Beatrica
Rezuchova, Bronislava
Kormanec, Jan
ARCHIVES OF MICROBIOLOGY, 2006, 186 (06) : 435 - 446
[10] Cascade of sigma factors in streptomycetes: identification of a new extracytoplasmic function sigma factor σJ that is under the control of the stress-response sigma factor σH in Streptomyces coelicolor A3(2)
Vladislava Mazurakova
Beatrica Sevcikova
Bronislava Rezuchova
Jan Kormanec
Archives of Microbiology, 2006, 186 : 435 - 446

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