Volumetric optoacoustic neurobehavioral tracking of epileptic seizures in freely-swimming zebrafish larvae

被引:3
|
作者
Ozsoy, Cagla [1 ,2 ,3 ]
Hotz, Adriana L. [4 ]
Rieser, Nicolas N. [4 ]
Chen, Zhenyue [1 ,2 ,3 ]
Dean-Ben, Xose Luis [1 ,2 ,3 ]
Neuhauss, Stephan C. F. [4 ]
Razansky, Daniel [1 ,2 ,3 ]
机构
[1] Univ Zurich, Fac Med, Inst Pharmacol & Toxicol, Zurich, Switzerland
[2] Univ Zurich, Inst Biomed Engn, Zurich, Switzerland
[3] Swiss Fed Inst Technol, Inst Biomed Engn, Dept Informat Technol & Elect Engn, Zurich, Switzerland
[4] Univ Zurich, Dept Mol Life Sci, Zurich, Switzerland
来源
FRONTIERS IN MOLECULAR NEUROSCIENCE | 2022年 / 15卷
基金
瑞士国家科学基金会; 欧洲研究理事会;
关键词
calcium imaging; fluorescence microscopy; optoacoustic tomography; photoacoustics; functional neuroimaging; epilepsy; Danio rerio; CELLULAR RESOLUTION; NEURAL ACTIVITY; MODELS; BEHAVIOR; DISORDERS;
D O I
10.3389/fnmol.2022.1004518
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Fast three-dimensional imaging of freely-swimming zebrafish is essential to understand the link between neuronal activity and behavioral changes during epileptic seizures. Studying the complex spatiotemporal patterns of neuronal activity at the whole-brain or -body level typically requires physical restraint, thus hindering the observation of unperturbed behavior. Here we report on real-time volumetric optoacoustic imaging of aberrant circular swimming activity and calcium transients in freely behaving zebrafish larvae, continuously covering their motion across an entire three-dimensional region. The high spatiotemporal resolution of the technique enables capturing ictal-like epileptic seizure events and quantifying their propagation speed, independently validated with simultaneous widefield fluorescence recordings. The work sets the stage for discerning functional interconnections between zebrafish behavior and neuronal activity for studying fundamental mechanisms of epilepsy and in vivo validation of treatment strategies.
引用
收藏
页数:9
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