Characterization of large yellow croaker (Larimichthys crocea) osteoprotegerin and its role in the innate immune response against to Vibrio alginolyticus

被引：3
|
作者：
Jin, Tian-Cheng [1 ,2 ,3 ]
Lu, Jian-Fei [1 ,2 ,3 ]
Luo, Sheng [1 ,2 ,3 ]
Wang, Li-Cong [1 ,2 ,3 ]
Lu, Xin-Jiang [1 ,2 ,3 ]
Chen, Jiong [1 ,2 ,3 ]
机构：
[1] Ningbo Univ, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China
[2] Ningbo Univ, Sch Marine Sci, Lab Biochem & Mol Biol, Ningbo 315211, Peoples R China
[3] Ningbo Univ, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Peoples R China
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2022年 / 258卷
基金：
中国国家自然科学基金;
关键词：
Larimichthys crocea; LcOPG; Vibrio alginolyticus; Monocytes/macrophages; MACROPHAGES; LIGAND; GENE; OSTEOCLASTS; APOPTOSIS; CYTOKINE; SYSTEM; FAMILY; BONE;
D O I：
10.1016/j.cbpb.2021.110680
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Osteoprotegerin (OPG) is a member of the tumor necrosis factor receptor superfamily, contributing to inflammation, apoptosis, and differentiation. However, the function of OPG in the host immune system of teleosts remains unclear. Here, we cloned the cDNA of the LcOPG gene from large yellow croaker. LcOPG mRNA was expressed in all analyzed tissues and was upregulated by Vibrio alginolyticus infection in immune tissues and monocytes/macrophages (MO/M Phi). Subsequently, the LcOPG protein was expressed and purified using a prokaryotic expression system. Recombinant LcOPG protein (rLcOPG) treatment suppressed V. alginolyticus-induced pro-inflammatory cytokine and enhanced V. alginolyticus-induced anti-inflammatory cytokine mRNA expression. Furthermore, rLcOPG decreased V. alginolyticus-induced MO/M Phi apoptosis. Therefore, the results indicate that LcOPG might play a role in the immune response of V. alginolyticus-infected large yellow croaker.
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页数：8
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