Crystallization and preliminary X-ray diffraction studies of a novel bacterial esterase

A novel bacterial esterase has been crystallized in two forms suitable for X-ray diffraction studies. Crystals have been obtained by vapour-phase diffusion at 290 K using ammonium sulfate as precipitant. The first crystals grew in space group C2 with unit-cell parameters a = 134.7, b = 55.8, c = 110.3 Angstrom, beta = 125.1 degrees. A monoclinic data set has been collected to 2.0 Angstrom resolution. Microseeding yielded a second crystal form which grew in space group P2(1)2(1)2(1) with unit-cell parameters a = 57.1, b = 115.4, c = 130.4 Angstrom. Native data from these crystals have been collected to 1.6 Angstrom resolution. A molecular envelope has been determined using an uranyl acetate derivative for phase calculation.