On allowing for transient variation in end-member δ13C values in partitioning soil C fluxes from net ecosystem respiration

The use of stable isotope analysis to resolve ecosystem respiration into its plant and soil components rests on how well the end-member isotope signatures (delta C-13) are characterised. In general, it is assumed that end-member values are constant over time. However, there are necessarily diurnal and other transient variations in end-members with environmental conditions. We analyse diurnal and seasonal patterns of ecosystem respiration and its delta C-13 in a C-4 grass growing in a C-3 soil using fixed and diurnally varying plant and soil delta C-13 end-members. We measure the end-members independently, and we assess the effects of expected variation in values. We show that variation in end-members within realistic ranges, particularly diurnal changes in the plant end-member, can cause partitioning errors of 40% during periods of high plant growth. The effect depends on how close the end-member is to the measured net respiration delta C-13, that is, the proportion of the respiration due to that end-member. We show light-driven variation in plant end-members can cause substantial distortion of partitioned soil organic matter (SOM) flux patterns on a diurnal scale and cause underestimation of daily to annual SOM turnover of approximately 25%. We conclude that, while it is not practicable to independently measure the full temporal variation in end-member values over a growing season, this error may be adjusted for by using a diurnally varying delta C-13(plant). Highlights End-member delta C-13 values used to partition ecosystem respiration vary diurnally and seasonally Patterns of ecosystem respiration and its delta C-13 in a C-4 grass growing in a C-3 soil were analysed. Ignoring temporal changes in end-member delta C-13 values can cause large errors in partitioning Long-term data sets with sufficient temporal resolution can be used to correct for this