Nerve Growth Factor Promotes the Survival of Goat Preantral Follicles Cultured in vitro

被引：27
|
作者：
Chaves, R. N. [1 ]
Alves, A. M. C. V. [1 ]
Duarte, A. B. G. [1 ]
Araujo, V. R. [1 ]
Celestino, J. J. H. [1 ]
Matos, M. H. T. [1 ]
Lopes, C. A. P. [1 ]
Campello, C. C. [1 ]
Name, K. P. O. [2 ]
Bao, S. N. [2 ]
Figueiredo, J. R. [1 ]
机构：
[1] Univ Estadual Ceara, Lab Manipulat Oocytes & Preantral Follicles, Fac Vet, Fortaleza, Ceara, Brazil
[2] Univ Brasilia, Lab Elect Microscopy, Dept Cell Biol, Brasilia, DF, Brazil
来源：
CELLS TISSUES ORGANS | 2010年 / 192卷 / 04期
关键词：
Goat; Ovarian follicles; Nerve growth factor; P75 NEUROTROPHIN RECEPTOR; PRIMORDIAL FOLLICLES; STIMULATING-HORMONE; RAT OVARY; EXPRESSION; TRKA; APOPTOSIS; BOVINE; PRESERVATION; ACTIVATION;
D O I：
10.1159/000317133
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
The aim of this study was to investigate the effects of nerve growth factor (NGF) on the in vitro culture of goat preantral follicles. Ovarian cortex fragments were cultured in alpha-MEM+ supplemented with 0, 1, 10, 50, 100 or 200 ng/ml NGF for 1 or 7 days. Small fragments of noncultured ovarian tissue as well as those cultured for 1 or 7 days were processed for histology and transmission electron microscopy. The results showed that after 1 or 7 days of culture at all concentrations of NGF, except at 1 ng/ml after 1 day of culture, there was a significant reduction in the percentage of normal follicles compared to noncultured tissues. At higher NGF concentrations (100 and 200 ng/ml) after 7 days of culture, there was a significant reduction in the percentage of normal follicles compared to tissues cultured in alpha-MEM+ alone or at the other concentrations of NGF. It is important to note that ultrastructural and fluorescent analyses confirmed only the integrity of follicles cultured with 1 ng/ml of NGF after 7 days. In contrast to noncultured control tissues, the percentage of developing follicles was significantly increased at all concentrations of NGF after 1 or 7 days of culture. We observed that follicular diameter was greater at 1 and 10 ng/ml NGF after culture for 7 days than at the other concentrations but was similar to follicles cultured in alpha-MEM+ alone. In conclusion, NGF improved the survival of goat preantral follicles cultured in vitro in a dose-dependent manner. Copyright (C) 2010 S. Karger AG, Basel
引用
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页码：272 / 282
页数：11
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