Synthesis of Biocompatible Chitosan-doped SiO2 Nanoparticles and Its Application for DNA Adsorption

被引：0
|
作者：
Zhang Lina [1 ]
Guo Zhihui [1 ]
Zheng Xingwang [1 ]
Wang Rong [1 ]
Meng Meirong [1 ]
Qu Yingjuan [1 ]
Liu Quanhong [2 ]
Yao Sha [1 ]
机构：
[1] Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci Shaanxi Prov, Xian 710062, Peoples R China
[2] Shaanxi Normal Univ, Coll Life Sci, Xian 710062, Peoples R China
来源：
ACTA CHIMICA SINICA | 2011年 / 69卷 / 20期
关键词：
chitosan-silica nanoparticles; reverse microemulsion; calf thymus DNA; SILICA NANOPARTICLES; DELIVERY;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel mesoporous chitosan-silica nanoparticles was synthesized with the water-in-oil (W/O) reverse microemulsion method. By characterizing this nanoparticles with transmission electron microscopy, IR spectra and the zeta potential experiments, it was found that those nanoparticles were spherical and uniform with 50 nm size and presented the mesoporous structure. Based on these findings, the adsorption ability of this nanoparticles for calf thymus DNA was studied. The relating results showed that this nanoparticles had a stronger adsorption ability to DNA in PBS buffer (pH=6), they also presented the better biocompatibility for cell and could be used for protect DNA from Dnase I cleavage.
引用
收藏
页码：2486 / 2492
页数：7
相关论文
共 21 条
  • [1] Surface modification of silica nanoparticles to reduce aggregation and nonspecific binding
    Bagwe, RP
    Hilliard, LR
    Tan, WH
    [J]. LANGMUIR, 2006, 22 (09) : 4357 - 4362
  • [2] Chitosan as a polymer for pH-induced DNA capture in a totally aqueous system
    Cao, Weidong
    Easley, Christopher J.
    Ferrance, Jerome P.
    Landers, James P.
    [J]. ANALYTICAL CHEMISTRY, 2006, 78 (20) : 7222 - 7228
  • [3] Plasmid DNA-entrapped nanoparticles engineered from microemulsion precursors: In vitro and in vivo evaluation
    Cui, ZR
    Mumper, RJ
    [J]. BIOCONJUGATE CHEMISTRY, 2002, 13 (06) : 1319 - 1327
  • [4] Adsorption of duplex DNA on mesoporous silicas: Possibility of inclusion of DNA into their mesopores
    Fujiwara, M
    Yamamoto, F
    Okamoto, K
    Shiokawa, K
    Nomura, R
    [J]. ANALYTICAL CHEMISTRY, 2005, 77 (24) : 8138 - 8145
  • [5] Synthesis of 6-N,N,N-Trimethyltriazole Chitosan via "Click Chemistry" and Evaluation for Gene Delivery
    Gao, Yu
    Zhang, Zhiwen
    Chen, Lingli
    Gu, Wangwen
    Li, Yaping
    [J]. BIOMACROMOLECULES, 2009, 10 (08) : 2175 - 2182
  • [6] Chitosan-Coated Silica as a Solid Phase for RNA Purification in a Microfluidic Device
    Hagan, Kristin A.
    Meier, Whitney L.
    Ferrance, Jerome P.
    Landers, James P.
    [J]. ANALYTICAL CHEMISTRY, 2009, 81 (13) : 5249 - 5256
  • [7] Bioconjugated nanoparticles for DNA protection from cleavage
    He, XX
    Wang, KM
    Tan, WH
    Liu, B
    Lin, X
    He, CM
    Li, D
    Huang, SS
    Li, J
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (24) : 7168 - 7169
  • [8] ILINA AV, 2005, BIOCH MICROBIOL, V41, P5
  • [9] Chitosan conjugated DNA nanoparticles in gene therapy
    Jayakumar, R.
    Chennazhi, K. P.
    Muzzarelli, R. A. A.
    Tamura, H.
    Nair, S. V.
    Selvamurugan, N.
    [J]. CARBOHYDRATE POLYMERS, 2010, 79 (01) : 1 - 8
  • [10] Jiang Tingda, 2001, CHITOSAN, P1
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