Easy to Use Plastic Optical Fiber-Based Biosensor for Detection of Butanal

被引：22

作者：

Cennamo, Nunzio ^{[1
]}

Di Giovanni, Stefano ^{[2
]}

Varriale, Antonio ^{[2
]}

Staiano, Maria ^{[2
]}

Di Pietrantonio, Fabio ^{[3
]}

Notargiacomo, Andrea ^{[4
]}

Zeni, Luigi ^{[1
]}

D'Auria, Sabato ^{[2
,5
]}

机构：

[1] SUN, Dept Ind & Informat Engn, Aversa, Italy

[2] CNR, Inst Prot Biochem, I-80125 Naples, Italy

[3] OM Corbino Inst Acoust & Sensors, Rome, Italy

[4] CNR, Inst Photon & Nanotechnol, Rome, Italy

[5] CNR, Inst Food Sci, Avellino, Italy

来源：

PLOS ONE | 2015年 / 10卷 / 03期

关键词：

ODORANT-BINDING-PROTEIN; SOLID-PHASE MICROEXTRACTION; SURFACE-PLASMON RESONANCE; STABILITY; MECHANISM; DERIVATIZATION; ALDEHYDES; DYNAMICS; SPR;

D O I：

10.1371/journal.pone.0116770

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The final goal of this work is to achieve a selective detection of butanal by the realization of a simple, small-size and low cost experimental approach. To this end, a porcine odorant-binding protein was used in connection with surface plasmon resonance transduction in a plastic optical fiber tool for the selective detection of butanal by a competitive assay. This allows to reduce the cost and the size of the sensing device and it offers the possibility to design a "Lab-on-a-chip" platform. The obtained results showed that this systema pproach is able to selectively detect the presence of butanal in the concentration range from 20 mu M to 1000 mu M.

引用

页数：12

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