Isolation and structural characterization of exopolysaccharide from marine Bacillus sp. and its optimization by Microbioreactor

被引：19
|
作者：
Wagh, Vasudev S. [1 ,3 ]
Said, Madhukar S. [2 ,3 ]
Bennale, Jagadish S. [4 ]
Dastager, Syed G. [1 ,3 ]
机构：
[1] CSIR, Natl Chem Lab, NCIM Resource Ctr, Pune 411008, Maharashtra, India
[2] Natl Chem Lab, CSIR, Div Organ Chem, Pune 411008, Maharashtra, India
[3] Acad Sci & Innovat Res AcSIR, Gaziabad 201002, India
[4] Beckman Coulter Life Sci, Mumbai 400070, Maharashtra, India
来源：
CARBOHYDRATE POLYMERS | 2022年 / 285卷
关键词：
Marine; EPS; Microbioreactor; Structure; Optimization; LEVAN PRODUCTION; POLYSACCHARIDE; PURIFICATION; ANTIOXIDANT; MEGATERIUM; FRUCTAN;
D O I：
10.1016/j.carbpol.2022.119241
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
In the present study, a cost-effective, robust Microbioreactor based production optimization of levan like exopolysaccharide from marine Bacillus sp. SGD-03 was analysed. FE-SEM analysis has showed the significant fibrillar structure of EPS. Size exclusion chromatography and other analytical data revealed that, produced EPS has a molecular weight of 1.0 x 10(4) Da and is composed of fructose monosaccharide with hydroxyl, carbonyl, and ether groups. The backbone structure of EPS has a branching pattern of beta-(2,6) linkages which confirms the similarity with available levan like polymers. The cost-effective media composition for levan production was demonstrated. The maximum yield of crude levan obtained was 123.9 g/L by response surface methodology using robust BioLector Pro Microbioreactor, and same has been validated with shake flask, 1 L and 10 L pilot-scale fermentation.
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页数：12
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