A study on preserving endometrial glandular architecture during preparation using BD SurePath™ liquid-based cytology reagents: Cellular fixation with preservative fluid requires at least 18 h

被引：1

作者：

Nishikawa, Takeshi ^{[1
]}

Suzuki, Hisae ^{[1
]}

Takeuchi, Mao ^{[1
]}

Tatsumi, Shigenobu ^{[1
]}

Tachibana, Yuma ^{[2
]}

Ohbayashi, Chiho ^{[3
]}

Kobayashi, Tadao K. ^{[4
]}

Norimatsu, Yoshiaki ^{[5
]}

机构：

[1] Nara Med Univ Hosp, Dept Pathol, Nara, Japan

[2] Yamatotakada Municipal Hosp, Dept Med Technol, Nara, Japan

[3] Nara Med Univ, Dept Diagnost Pathol, Sch Med, Nara, Japan

[4] Osaka Univ, Canc Educ & Res Ctr, Grad Sch Med, Osaka, Japan

[5] Ehime Prefectural Univ Hlth Sci, Fac Hlth Sci, Dept Med Technol, 543 Tobe Cho, Tobe, Ehime, Japan

来源：

CYTOPATHOLOGY | 2022年 / 33卷 / 03期

关键词：

cellular fixation; endometrial cytology; endometrial glandular architecture; liquid based cytology; preservative fluid;

D O I：

10.1111/cyt.13087

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Introduction This study aimed to determine the causes of disruption of the three-dimensional architecture of endometrial glands prepared using BD SurePath (TM) liquid-based cytology (SP-LBC) reagents. One sample preparation method for endometrial cytology is presented in which this three-dimensional architecture can be retained. Methods SP-LBC specimens were prepared by the following three methods: (1) using the BD PrepMate(TM) (PrepMate) System after cellular fixation for 1-6 h (method A); (2) without using the PrepMate System after cellular fixation for 1-6 h (method B); and (3) using the PrepMate System after cellular fixation for at least 18 h (method C). Size and numbers of endometrial cell clusters and numbers of solitary scattered cells were then evaluated. Results Significantly higher numbers of cell clusters with a major axis of 200 mu m or more were yielded by method C (71.3 +/- 57.2) than methods A (9.3 +/- 5.9, P < 0.001) or B (44.3 +/- 28.8, P < 0.05). Method B yielded significantly higher numbers of cell clusters than method A (P < 0.001). Method A (132.2 +/- 107.7, p < 0.001) yielded significantly higher numbers of solitary scattered cells than methods B (29.1 +/- 14.8) and C (35.7 +/- 23.3). No significant difference in solitary cell numbers was found between methods B and C. Conclusions Retention of endometrial glandular architecture is rendered possible by allowing sample fixation times of 18 h or more when preparing specimens using the PrepMate System.

引用

页码：357 / 361

页数：5