A sensitive and specific lateral flow assay for rapid detection of antibodies against glycoprotein B of Aujeszky's disease virus

被引：12
|
作者：
Vrublevskaya, Veronika V. [1 ]
Afanasyev, Vladimir N. [1 ]
Grinevich, Andrey A. [1 ]
Skarga, Yuri Y. [1 ]
Gladyshev, Pavel P. [2 ]
Ibragimova, Sagila A. [2 ]
Krylsky, Dmitry V. [3 ]
Dezhurov, Sergey V. [3 ]
Morenkov, Oleg S. [1 ]
机构：
[1] Russian Acad Sci, Inst Cell Biophys, Pushchino 142290, Moscow Region, Russia
[2] State Univ Dubna, Dubna 141982, Moscow Region, Russia
[3] Res Inst Appl Acoust, Dubna 141980, Moscow Region, Russia
来源：
JOURNAL OF VIROLOGICAL METHODS | 2017年 / 249卷
基金：
俄罗斯科学基金会;
关键词：
Aujeszky's disease virus; Lateral flow assay; Antibody detection; PSEUDORABIES VIRUS; STRIP; GII;
D O I：
10.1016/j.jviromet.2017.09.012
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A direct double antibody lateral flow assay (DDA-gB-LFA) for the detection of antibodies against the glycoprotein B (gB) of Aujeszky's disease virus (ADV) in swine sera was developed. A native ADV gB was used for the preparation of a conjugate with colloidal gold particles and the immobilization on the strip membrane. The gB purified from ADV virions by immunoaffinity chromatography retained its native epitope structure after adsorption on the nitrocellulose membrane and the surface of colloidal gold particles. The diagnostic specificity and sensitivity of the DDA-gB-LFA were evaluated using 236 field swine sera. The diagnostic specificity and sensitivity of the DDA-gB-LFA compared to a commercially available gB-based ELISA were 98.0% and 98.6%, respectively, when determined with the use of the reader-detection mode, and 98.0% and 93.5%, respectively, when determined using visual detection. The DDA-gB-LFA provides a rapid, sensitive, and specific determination of ADV gB-directed antibodies in sera and can be used for the detection of ADV-exposed swine.
引用
收藏
页码：175 / 180
页数：6
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