DNA double-strand break misrejoining after exposure of primary human fibroblasts to CK characteristic X rays, 29 kVp X rays and 60Co γ rays

被引:33
|
作者
Kühne, M
Urban, G
Frankenberg, D [1 ]
Löbrich, M
机构
[1] Univ Saarland, Dept Biophys, Fachrichtung Biophys, D-66421 Homburg, Germany
[2] Univ Gottingen, Klin Strahlenbiol & Clin Strahlenphys, D-37075 Gottingen, Germany
关键词
D O I
10.1667/RR3461.1
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The efficiency of ionizing photon radiation for inducing mutations, chromosome aberrations, neoplastic cell transformation, and cell killing depends on the photon energy. We investigated the induction and rejoining of DNA double-strand breaks (DSBs) as possible contributors for the varying efficiencies of different photon energies. A specialized pulsed-field gel electrophoresis assay based on Southern hybridization of single Mbp genomic restriction fragments was employed to assess DSB induction and rejoining by quantifying the restriction fragment band. Unrejoined and misrejoined DSBs were determined in dose fractionation protocols using doses per fraction of 2.2 and 4.4 Gy for C-K characteristic X rays, 4 and 8 Gy for 29 kVp X rays, and 5, 10 and 20 Gy for Co-60 gamma rays. DSB induction by C-K characteristic X rays was about twofold higher than for Co-60 gamma rays, whereas 29 kVp X rays showed only marginally elevated levels of induced DSBs compared with Co-60 gamma rays (a factor of 1.15). Compared with these modest variations in DSB induction, the variations in the levels of unrejoined and misrejoined DSBs were more significant. Our results suggest that differences in the fidelity of DSB rejoining together with the different efficiencies for induction of DSBs can explain the varying biological effectiveness of different photon energies.
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收藏
页码:669 / 676
页数:8
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