Modified binary hammerhead ribozymes with high catalytic activity

被引：1

作者：

Vorobjeva, M ^{[1
]}

Gusseva, E ^{[1
]}

Repkova, M ^{[1
]}

Kovalev, N ^{[1
]}

Zenkova, M ^{[1
]}

Venyaminova, A ^{[1
]}

Vlassov, V ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Chem Biol & Fundamental Med, SB, Novosibirsk 630090, Russia

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2005年 / 24卷 / 5-7期

关键词：

RNA cleavage; hammerhead ribozyme; modified binary ribozyme; nuclease resistance; MDR1; mRNA;

D O I：

10.1081/NCN-200060181

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of binary hammerhead ribozymes was designed and assessed in terms of cleavage activity and nuclease resistance. Enhanced nuclease resistance of binary ribozymes was achieved by incorporation of 2'-modified nucleotides at the selective positions along with addition of 3'-3'-linked thymidine cap. These modified binary ribozymes efficiently cleave 190-nucleotides long MDR1 mRNA fragment and display catalytic activity much higher then respective full-length analogs.

引用

页码：1105 / 1109

页数：5

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