X-ray fluorescence microscopy methods for biological tissues

被引:17
|
作者
Pushie, M. Jake [1 ]
Sylvain, Nicole J. [1 ,2 ]
Hou, Huishu [1 ]
Hackett, Mark J. [3 ,4 ]
Kelly, Michael E. [1 ]
Webb, Samuel M. [5 ]
机构
[1] Univ Saskatchewan, Coll Med, Dept Surg, Div Neurosurg, Saskatoon, SK S7N 5E5, Canada
[2] Univ Saskatchewan, Coll Med, Clin Trial Support Unit, Saskatoon, SK S7N 0W8, Canada
[3] Curtin Univ, Curtin Hlth Innovat Res Inst, Perth, WA 6102, Australia
[4] Curtin Univ, Sch Mol & Life Sci, Perth, WA 6845, Australia
[5] SLAC Natl Accelerator Lab, Stanford Synchrotron Radiat Lightsource, Mail Stop 69,2575 Sand Hill Rd, Menlo Pk, CA 94025 USA
基金
美国国家卫生研究院;
关键词
X-ray fluorescence microscopy; mapping; imaging; XFI; XFM; biological samples; chemical speciation; ALZHEIMERS-DISEASE; K-EDGE; COPPER; MOUSE; TRANSMISSION; SCRAPIE; IRON; CU; MICROANALYSIS; MECHANISMS;
D O I
10.1093/mtomcs/mfac032
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Synchrotron-based X-ray fluorescence microscopy is a flexible tool for identifying the distribution of trace elements in biological specimens across a broad range of sample sizes. The technique is not particularly limited by sample type and can be performed on ancient fossils, fixed or fresh tissue specimens, and in some cases even live tissue and live cells can be studied. The technique can also be expanded to provide chemical specificity to elemental maps, either at individual points of interest in a map or across a large field of view. While virtually any sample type can be characterized with X-ray fluorescence microscopy, common biological sample preparation methods (often borrowed from other fields, such as histology) can lead to unforeseen pitfalls, resulting in altered element distributions and concentrations. A general overview of sample preparation and data-acquisition methods for X-ray fluorescence microscopy is presented, along with outlining the general approach for applying this technique to a new field of investigation for prospective new users. Considerations for improving data acquisition and quality are reviewed as well as the effects of sample preparation, with a particular focus on soft tissues. The effects of common sample pretreatment steps as well as the underlying factors that govern which, and to what extent, specific elements are likely to be altered are reviewed along with common artifacts observed in X-ray fluorescence microscopy data.
引用
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页数:31
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