Retention and Separation Efficiency of Some Synthetic Oligopeptides in Reversed-Phase Thin-Layer Chromatography

被引：11
|
作者：
Gwarda, R. L. [1 ]
Tomczyszyn, A. [2 ]
Misicka, A. [2 ]
Dzido, T. H. [1 ]
机构：
[1] Med Univ Lublin, Dept Phys Chem, Chair Chem, PL-20093 Lublin, Poland
[2] Univ Warsaw, Fac Chem, PL-02093 Warsaw, Poland
来源：
ACTA CHROMATOGRAPHICA | 2015年 / 27卷 / 01期
关键词：
peptide retention; peptide separation; reversed-phase chromatography; thin-layer chromatography; TRYPTIC PROTEIN DIGESTS; IONIZATION MASS-SPECTROMETRY; HPTLC/DESI-MS; PEPTIDES; SENSITIVITY;
D O I：
10.1556/AChrom.27.2015.1.1
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Influence of the following variables such as adsorbent type, type and concentration of organic modifier in mobile phase, type and concentration of ion-pairing reagent, or pH of the mobile phase buffer on retention of some synthetic peptides in reversed-phase high-performance thin-layer chromatography systems has been investigated. The investigations have been also focused on influence of the variables mentioned on solute zone shape regarding optimization of their separation. Remarks about solute retention mechanism have been also provided.
引用
收藏
页码：1 / 14
页数：14
相关论文
共 50 条
  • [1] REVERSED-PHASE THIN-LAYER CHROMATOGRAPHY FOR THE SEPARATION AND ANALYSIS OF ECDYSTEROIDS
    WILSON, ID
    SCALIA, S
    MORGAN, ED
    JOURNAL OF CHROMATOGRAPHY, 1981, 212 (02): : 211 - 219
  • [2] IMPROVED SEPARATION OF STEROLS BY REVERSED-PHASE THIN-LAYER CHROMATOGRAPHY
    DESOUZA, NJ
    NES, WR
    JOURNAL OF LIPID RESEARCH, 1969, 10 (02) : 240 - &
  • [3] RETENTION BEHAVIOR OF SOME AMIDES IN REVERSED-PHASE AND NORMAL-PHASE THIN-LAYER CHROMATOGRAPHY
    PERISICJANJIC, NU
    PETROVIC, SM
    DJAKOVIC, T
    NIKOLIC, AD
    PETROVIC, SD
    CHROMATOGRAPHIA, 1992, 34 (9-10) : 537 - 539
  • [4] Retention behavior of some thiophosphorylglycinamide fungicides in adsorption and reversed-phase thin-layer chromatography
    Oros, Gy.
    Cserhati, T.
    Illes, Z.
    JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2006, 29 (14) : 2009 - 2018
  • [5] NORMAL AND REVERSED-PHASE THIN-LAYER CHROMATOGRAPHY OF SOME AZOLIDONES
    PETROVIC, SM
    LONCAR, E
    PERISICJANJIC, NU
    POPOVPERGAL, K
    CHROMATOGRAPHIA, 1994, 38 (11-12) : 744 - 748
  • [6] Reversed-Phase Thin-Layer Chromatography of Some Foodstuff Dyes
    Dusanka M. Milojković-Opsenica
    Kristina Lazarević
    Vojkan Ivačković
    Zivoslav Lj. Tešić
    JPC – Journal of Planar Chromatography – Modern TLC, 2003, 16 : 276 - 279
  • [7] REVERSED-PHASE THIN-LAYER CHROMATOGRAPHY OF SOME COMMON STEROLS
    DOMNAS, AJ
    WARNER, SA
    JOHNSON, SL
    LIPIDS, 1983, 18 (01) : 87 - 89
  • [8] Reversed-phase thin-layer chromatography of some foodstuff dyes
    Milojkovic-Opsenica, DM
    Lazarevic, K
    Ivackovic, V
    Tesic, ZL
    JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC, 2003, 16 (04) : 276 - 279
  • [9] EFFECT OF MODIFIER ON THE RETENTION OF SOLUTES IN REVERSED-PHASE THIN-LAYER CHROMATOGRAPHY
    BIEGANOWSKA, M
    SOCZEWINSKI, E
    WAKSMUNDZKAHAJNOS, M
    JOURNAL OF LIQUID CHROMATOGRAPHY, 1985, 8 (07): : 1207 - 1224
  • [10] SEPARATION AND IDENTIFICATION OF IRIDOID GLYCOSIDES BY REVERSED-PHASE THIN-LAYER CHROMATOGRAPHY
    ORSINI, F
    PELIZZONI, F
    SELLO, G
    ANNALI DI CHIMICA, 1982, 72 (11-1) : 635 - 637
12345