MUC1 Aptamer-Based Near-Infrared Fluorescence Probes for Tumor Imaging

被引：28
|
作者：
Chen, Haiyan [1 ]
Zhao, Juan [1 ]
Zhang, Min [1 ]
Yang, Haibo [2 ]
Ma, Yuxiang [1 ]
Gu, Yueqing [1 ]
机构：
[1] China Pharmaceut Univ, State Key Lab Nat Med, Sch Life Sci & Technol, Dept Biomed Engn, Nanjing 210009, Peoples R China
[2] China Pharmaceut Univ, Sch Pharm, Nanjing 210009, Peoples R China
来源：
MOLECULAR IMAGING AND BIOLOGY | 2015年 / 17卷 / 01期
关键词：
MUC1; aptamer; Tumor targeting; PEG; Near-infrared fluorescence; Tumor imaging; IN-VIVO; CANCER; NANOPARTICLE; NUCLEOLIN; MUCINS; DYES;
D O I：
10.1007/s11307-014-0763-y
中图分类号：
R8 [特种医学]; R445 [影像诊断学];
学科分类号：
1002 ; 100207 ; 1009 ;
摘要：
Purpose: DNA aptamer (APT) is able to bind to Mucin 1 (MUC1) specifically. The possibility of APT acting as a moiety to construct tumor-targeting probes was investigated. Procedures: A near-infrared (NIR) fluorescent dye (MPA) and polyethylene glycol (PEG) were conjugated to APT to form APT-MPA and APT-PEG-MPA. The successful synthesis of the two probes was characterized via thin layer chromatography (TLC) and optical spectra. The tumor-targeting efficacy of the probes was evaluated in detail at cell level and animal level, respectively. Results: The results indicated that MPA and PEG were successfully coupled with APT. APT-based probes were mediated by Mucin 1 into tumor cells, and PEG-modified probe exhibited higher cell affinity. Conclusions: The aptamer-based NIR fluorescent probes are promising candidates for tumor imaging and diagnosis.
引用
收藏
页码：38 / 48
页数：11
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