Electrochemical biosensor based on nanoporous gold electrode for detection of PML/RARα fusion gene

被引：42
|
作者：
Zhong, Guangxian [2 ]
Liu, Ailin [1 ,2 ]
Chen, Xuhai [3 ,6 ]
Wang, Kun [4 ]
Lian, Zhixian [2 ]
Liu, Qicai [5 ]
Chen, Yuanzhong [1 ,2 ]
Du, Min [3 ]
Lin, Xinhua [2 ]
机构：
[1] Fujian Med Univ, Affiliated Union Hosp, Fujian Inst Hematol, Fuzhou 350000, Peoples R China
[2] Fujian Med Univ, Dept Pharmaceut Anal, Fac Pharm, Fuzhou 350004, Peoples R China
[3] Fuzhou Univ, Fujian Key Lab Med Instrumentat & Pharmaceut Tech, Fuzhou 350002, Fujian, Peoples R China
[4] Xiamen Univ, Affiliated Hosp 1, Dept Pharm, Xiamen 361003, Peoples R China
[5] Fujian Med Univ, Affiliated Hosp 1, Dept Clin Lab, Fuzhou 350004, Peoples R China
[6] Fuzhou Univ, Coll Elect Engn & Automat, Fuzhou 350000, Peoples R China
来源：
BIOSENSORS & BIOELECTRONICS | 2011年 / 26卷 / 09期
基金：
中国国家自然科学基金;
关键词：
Nanoporous gold electrode; Square-wave oxidation reduction cycle; DNA biosensors; PML/RAR alpha fusion gene; Methylene Blue; ACUTE PROMYELOCYTIC LEUKEMIA; HEPATITIS-B-VIRUS; METHYLENE-BLUE; PLATINUM-ELECTRODES; MOLECULAR ANALYSIS; DNA; METAL; HYBRIDIZATION; NANOPARTICLES; DIAGNOSIS;
D O I：
10.1016/j.bios.2011.02.039
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
In this study, a kind of nanoporous gold electrode (NPG) prepared with repetitive square-wave oxidation reduction cycle (SWORC) was reported. The active surface area of the proposed NPG electrode was 9.9 times larger than that of a bare flat one characterized by cyclic voltammetry (CV). An electrochemical DNA biosensor based on NPG electrode was fabricated for detection of promyelocytic leukemia/retinoic acid receptor alpha (PML/RAR alpha) fusion gene in acute promyelocytic leukemia (APL) by using Methylene Blue (MB) as an electroactive indicator. Differential pulse voltammetry (DPV) was employed to monitor the hybridization reaction on the probe modified electrode. The decrease of the peak current of MB was observed upon hybridization of the probe with target DNA. The results indicated that the peak current was linear with the concentration of complementary strand in the range of 60 pM to 220 pM with a detection limit of 6.7 pM. This new biosensor exhibited excellent sensitivity and selectivity and had been used for an assay of PCR real sample with a satisfactory result. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：3812 / 3817
页数：6
相关论文
共 50 条
  • [1] Electrochemical biosensor for detection of PML/RARα fusion gene based on eriochrome cyanine R film modified glassy carbon electrode
    Wang, Liman
    Lin, Liqing
    Xu, Xiongwei
    Weng, Shaohuang
    Lei, Yun
    Liu, Ailin
    Chen, Yuanzhong
    Lin, Xinhua
    [J]. ELECTROCHIMICA ACTA, 2012, 69 : 56 - 59
  • [2] Enzyme-amplified electrochemical biosensor for detection of PML-RARα fusion gene based on hairpin LNA probe
    Lin, Liging
    Liu, Qicai
    Wang, Liman
    Liu, Ailin
    Weng, Shaohuang
    Lei, Yun
    Chen, Wei
    Lin, Xinhua
    Chen, Yuanzhong
    [J]. BIOSENSORS & BIOELECTRONICS, 2011, 28 (01): : 277 - 283
  • [3] Design of a sandwich-mode amperometric biosensor for detection of PML/RARα fusion gene using locked nucleic acids on gold electrode
    Wang, Kun
    Sun, Zhouliang
    Feng, Meijuan
    Liu, Ailin
    Yang, Shuyu
    Chen, Yuanzhong
    Lin, Xinhua
    [J]. BIOSENSORS & BIOELECTRONICS, 2011, 26 (06): : 2870 - 2876
  • [4] Efficient Determination of PML/RARα Fusion Gene by the Electrochemical DNA Biosensor Based on Carbon Dots/Graphene Oxide Nanocomposites
    Zhang, Zi-Yang
    Huang, Lin-Xiao
    Xu, Zhi-Wei
    Wang, Peng
    Lei, Yun
    Liu, Ai-Lin
    [J]. INTERNATIONAL JOURNAL OF NANOMEDICINE, 2021, 16 : 3497 - 3508
  • [5] Electrochemical DNA biosensor based on aldehyde-agarose hydrogel modified glassy carbon electrode for detection of PML/RARa fusion gene
    Liu, Ailin
    Chen, Xuhai
    Wang, Kun
    Wei, Na
    Sun, Zhouliang
    Lin, Xinhua
    Chen, Yuanzhong
    Du, Min
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 2011, 160 (01): : 1458 - 1463
  • [6] Electrochemical Biosensor Based on Hierarchical Nanoporous Composite Electrode for Detection of Oxytetracycline
    Du, Jingjing
    Song, Ya
    Xie, Shaowen
    Feng, Yahui
    Jiang, Jianbing
    Xu, Lijian
    [J]. NANOSCIENCE AND NANOTECHNOLOGY LETTERS, 2018, 10 (08) : 1095 - 1100
  • [7] An electrochemical NADH biosensor based on a nanoporous gold electrode modified with diaphorase and an osmium polymer
    Otero, Fernando
    Mandal, Tanushree
    Leech, Donal
    Magner, Edmond
    [J]. SENSORS AND ACTUATORS REPORTS, 2022, 4
  • [8] Nanoporous gold electrode as a platform for the construction of an electrochemical DNA hybridization biosensor
    Ahangar, Laleh Enayati
    Mehrgardi, Masoud A.
    [J]. BIOSENSORS & BIOELECTRONICS, 2012, 38 (01): : 252 - 257
  • [9] Design of a Solid-State Electrochemiluminescence Biosensor for Detection of PML/RAR Fusion Gene Using Ru(bpy)${{{2+\hfill \atop 3\hfill}}}$-AuNPs Aggregations on Gold Electrode
    Lei, Yun
    Lin, Yajing
    Zheng, Yanjie
    Dai, Ming
    Wang, Kun
    Lin, Xinhua
    [J]. ELECTROANALYSIS, 2013, 25 (06) : 1388 - 1394
  • [10] A simple surface plasmon resonance biosensor for detection of PML/RARα based on heterogeneous fusion gene-triggered nonlinear hybridization chain reaction
    Guo, Bin
    Cheng, Wei
    Xu, Yongjie
    Zhou, Xiaoyan
    Li, Xinmin
    Ding, Xiaojuan
    Ding, Shijia
    [J]. SCIENTIFIC REPORTS, 2017, 7
12345