ZnO, TiO2, and CuO nanoparticles were immobilized onto the cotton and wool fabrics at room temperature. The antimicrobial activity of dyed cotton and wool fabrics with reactive dye against S-aureus, E-coli, and C-candida were measured by the optical density method. It was observed that the killing of bacteria and the bacterial cell damage were harmed efficiently. The effect of pre-treatment of cotton and wool fabrics with different NPs metal oxides on the color strength of dyed fabrics was studied. Color strength (K/S) and colorimetric data (L*, a*, and b*) were measured at specific lambda max. Besides, washing, rubbing, perspiration, and lightfastness of dyed cotton and wool fabrics were determined. UV blocking and self-cleaning of the treated fabrics were measured accordingly. The obtained results of the pre-treated fabrics with ZnO, TiO2, and CuO nanoparticles would support the current research outputs as a potential production of protective textile.